This product is supplied as a 100ug/ml solution in ethanol. The recommended storage temperature is -20°C. The item is not assigned an expiration date, but a warranty of 1 year from the date of shipment is applied. Solutions of Vitamin D3 prepared from the powder form are vulnerable to degradation by exposure to light and air. For additional details regarding the stability of this material in ethanol, please review the sample Certificate of Analysis below, page 9.
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/cer/V-025_FN05252107.pdf
740284
Vitamin D3(6,19,19-d3) solution
100 μg/mL in ethanol, 97 atom % D, 97% (CP)
Synonym(s):
Calciol (6,19,19-d3) solution, Cholecalciferol (6,19,19-d3) solution
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About This Item
Empirical Formula (Hill Notation):
C27D3H41O
CAS Number:
Molecular Weight:
387.66
NACRES:
NA.24
PubChem Substance ID:
UNSPSC Code:
12352106
EC Number:
200-578-6
MDL number:
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Product Name
Vitamin D3(6,19,19-d3) solution, 100 μg/mL in ethanol, 97 atom % D, 97% (CP)
InChI key
QYSXJUFSXHHAJI-GLSUUORTSA-N
InChI
1S/C27H44O/c1-19(2)8-6-9-21(4)25-15-16-26-22(10-7-17-27(25,26)5)12-13-23-18-24(28)14-11-20(23)3/h12-13,19,21,24-26,28H,3,6-11,14-18H2,1-2,4-5H3/b22-12+,23-13-/t21-,24+,25-,26+,27-/m1/s1/i3D2,13D
SMILES string
[2H]/C([2H])=C1CC[C@H](O)CC\1=C([2H])\C=C2/CCC[C@]3(C)[C@H](CC[C@@H]23)[C@H](C)CCCC(C)C
isotopic purity
97 atom % D
assay
97% (CP)
form
solution
concentration
100 μg/mL in ethanol
technique(s)
mass spectrometry (MS): suitable
color
colorless
application(s)
detection
mass shift
M+3
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | 731285 | 739847 | 739596 |
|---|---|---|---|
| technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable |
| assay 97% (CP) | assay 97% (CP) | assay 98% (CP) | assay 98% (CP) |
| mass shift M+3 | mass shift M+3 | mass shift M+3 | mass shift M+3 |
| isotopic purity 97 atom % D | isotopic purity 97 atom % D | isotopic purity 97 atom % D | isotopic purity 97 atom % D |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form liquid | form solution | form liquid |
Application
Deuterated vitamin D3(6,19,19-d3) solution is also known as cholecalciferol (6, 19, 19-d3). It can be used as a stable isotope internal standard for the quantification of vitamin D in biological samples by a liquid chromatography-tandem mass spectrometry (LC-MS/MS).[1][2]
This product may be used as an analytical standard.
Packaging
1 mL in ampule
related product
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
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Potential intake of vitamins ?A? and ?D? through branded intravenous lipid emulsions: Liquid Chromatography-Tandem Mass Spectrometry Analysis
Forchielli ML, et al.
Clinical Nutrition (Edinburgh, Scotland), 36(2), 530-536 (2017)
Mechanism of error caused by isotope-labeled internal standard: accurate method for simultaneous measurement of vitamin D and pre-vitamin D by liquid chromatography/tandem mass spectrometry
Huang M, et al.
Rapid Communications in Mass Spectrometry, 28(19), 2101-2110 (2014)
Linda Ahonen et al.
Journal of chromatography. A, 1364, 214-222 (2014-09-11)
We have developed an ultra-high-performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometric (UHPLC-APPI-MS/MS) method for the simultaneous quantitative analyses of several oxysterols and vitamin D metabolites in mouse brain and cell line samples. An UHPLC-APPI-high resolution mass spectrometric (UHPLC-APPI-HRMS) method that
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I have been using vitamin D powder in ethanol to treat my cells. How long can I store this solution in -20?
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