Due to repulsive forces arising from the surface charge of gold nanoparticles, an energy barrier must be overcome for individual particles to interact. When no (or small) amounts of electrolytes such as NaCl is present, this energy barrier is too strong for interaction to occur between particles. However, upon addition of NaCl this energy barrier is reduced allowing the gold nanoparticles to interact and aggregate. This aggregation causes a phenomenon called surface-plasmon coupling which changes the adsorption maximum of light to a higher wavelength resulting in a change in color of the solution.
742007
Gold nanoparticles
50 nm diameter, OD 1, suspension in citrate buffer
Synonym(s):
Au NP, Gold Colloid
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25 ML
$144.00
100 ML
$458.00
About This Item
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form
nanoparticles
suspension
concentration
~3.5E+10 particles/mL
OD
1
diameter
50 nm
λmax
531-535 nm
PDI
<0.2
storage temp.
2-8°C
SMILES string
[Au]
InChI
1S/Au
InChI key
PCHJSUWPFVWCPO-UHFFFAOYSA-N
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This Item | 742090 | 742066 | 742015 |
|---|---|---|---|
| form nanoparticles, suspension | form nanoparticles, suspension | form nanoparticles, suspension | form nanoparticles, suspension |
| contains Proprietary Surfactant as stabilizer | contains Proprietary Surfactant as stabilizer | contains Proprietary Surfactant as stabilizer | contains Proprietary Surfactant as stabilizer |
| concentration ~3.5E+10 particles/mL | concentration ~1.9E+8 particles/mL | concentration ~1.9E+9 particles/mL | concentration ~1.9E+10 particles/mL |
| OD 1 | OD - | OD - | OD 1 |
| diameter 50 nm | diameter 400 nm | diameter 200 nm | diameter 60 nm |
| λmax 531-535 nm | λmax - | λmax - | λmax 536-541 nm |
Application
Ideal for adsorption of thiolated ligands such as oligonucleotides and polyethylene glycols. Also, due to the increased stability, these gold nanoparticles are ideal for use in physical and optical applications. Note: This product is not ideal for protein adsorption. For gold nanoparticles suitable for adsorption of proteins and other ligands for conjugate development, please see our gold nanoparticles stabilized in PBS.
This material is highly monodisperse (<12% variability in size and shape), and provides significantly improved surface reactivity. Applications include Surface Enhanced Raman Lables, Sensing/Detection, Biological Targeting, Plasmonics and Electronics.
This material is highly monodisperse (<12% variability in size and shape), and provides significantly improved surface reactivity. Applications include Surface Enhanced Raman Lables, Sensing/Detection, Biological Targeting, Plasmonics and Electronics.
Legal Information
Product of CytoDiagnostics, Inc.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Why does my gold nanoparticle solution turn violet when I add salt containing buffer?
1 answer-
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What is the shape of the gold nanoparticles?
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The majority (>95%) of the gold nanoparticles are spherical.
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How do you attach gold nanoparticles to oligos?
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To attach gold nanoparticles to oligos please view the information in the attached reference - Bioconjug Chem. 2011 Apr 20;22(4):794-807. Epub 2011 Mar 28.
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How do you attach gold nanoparticles to peptides and peptide conjugates?
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To view information on how to attach gold nanoparticles to peptides and peptide conjugates, please view the information in the reference - Rosenthal, S. J. and Wright, D. W. (Eds.). (2005), p. 91-92. NanoBiotechnology Protocols. New Jersey. Humana Press.
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Do I need to wash my gold nanoparticles?
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For most applications, our gold nanoparticles can be used without any additional washing steps. If you have a sensitive application that requires additional washing the best way to do so is by either centrifugation or filtration.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can the Gold nanoparticles be frozen?
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No, the Gold nanoparticles should not be frozen. If frozen, the gold nanoparticles will irreversibly aggregate, turning the solution clear.
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My gold nanoparticles settle out of solution upon storage. Is this normal?
1 answer-
Settling of gold nanoparticles on the bottom of the storage flask is completely normal and is especially common for larger sized particles, which settles at greater speed. Settling does not affect the performance of the particles. Prior to use, simply swirl the solution to properly disperse your gold nanoparticles and obtain a homogenous solution.
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Which gold nanoparticle size should I choose for my application?
1 answer-
The size of gold nanoparticle to use is very depandent upon the intended application. Generally, smaller particles offer better sensitivity in applications such as immunogold labeling due to less steric hindrance and the ability to bind more gold nanoparticles to the desired target. Small gold nanoparticles are less visible than larger particles, however, which must also be taken into account.As an application example, particles with a size between 30-50nm are particularly useful for the development of rapid tests such as lateral flow assays.
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How do you attach gold nanoparticles to glass?
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To view the procedure on how to attach gold nanoparticles to glass please view information in the reference - Journal of Atomic, Molecular, and Optical PhysicsVolume 2012 (2012). Article ID 683830, 6 pagesdoi:10.1155/2012/683830
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