These 5 nm gold nanoparticles are indeed suitable for developing peptide and protein gold conjugates, and they are commonly used in applications such as blotting, lateral flow assays, microscopy, and transmission electron microscopy (TEM). However, they have not been specifically tested for CryoElectron tomography.
Regarding affinity with proteins, including Amyloid beta peptides, while gold nanoparticles can interact with various biomolecules, the specific affinity would depend on the surface chemistry and functionalization of the nanoparticles.
752568
Gold nanoparticles
5 nm diameter, OD 1, suspension in 0.1 mM PBS, reactant free
Synonym(s):
Au NP, Gold colloid
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25 ML
$149.40
100 ML
$472.50
About This Item
$149.40
List Price$166.00Save 10%Web-Only Promotion
Available to ship onMay 20, 2025Details
Request a Bulk Order
Recommended Products
Quality Level
form
nanoparticles
suspension
concentration
~5.5E+13 particles/mL
OD
1
diameter
5 nm
λmax
510-520 nm
storage temp.
2-8°C
SMILES string
[Au]
InChI
1S/Au
InChI key
PCHJSUWPFVWCPO-UHFFFAOYSA-N
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General description
Goldnanoparticles with size of 5 nm and polydispersity index of <0.2 offer unique characteristics and have diverse applications in various industries. PBS maintains a physiological pH and prevents aggregation of nanoparticles. Smaller nanoparticles exhibit unique optical properties, including a strong surface plasmon resonance (SPR), which can be utilized in imaging and sensing applications. Small size also allows for enhanced permeability and retention effect in tumors, making them effective carriers for targeted drug delivery.
Application
- Gold nanoparticles: Synthesis properties and applications: A comprehensive overview of the methods for synthesizing gold nanoparticles and their broad range of applications (I Hammami, NM Alabdallah, 2021).
Legal Information
Product of CytoDiagnostics, Inc.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Are these 5nm gold nanoparticles suitable for CryoElectron tomography sample preparation. Can you please tell if they have any sort of affinity with the protein particularly Amyloid beta peptides.
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What is the composition and pH of 0.1mM PBS?
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The pH of this product is not determined. Based on the product page, the only listed components are Gold nanoparticles in 0.1 mM phosphate-buffered saline (PBS). Any other components are considered proprietary.
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Could you please confirm whether the nanoparticles are sterile and are suitable for cell-based experiments? If not sterile, what recommended sterilization method should I use to ensure their sterility?
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This product is not sterile. Please see the links below to publications that offer information on sterilization of gold nanoparticles:
https://pubs.acs.org/doi/10.1021/acs.langmuir.2c01557#:~:text=3.3.-,1.,C%20for%20about%2020%20min.
https://pubs.acs.org/doi/10.1021/acs.langmuir.2c01557
https://pubmed.ncbi.nlm.nih.gov/19937607/Helpful?
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What is the Z potential of the nanoparticles?
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The product in question has a net negative charge with a zeta potential in the range of –20mV to –30mV.
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What is the shape of the gold nanoparticles?
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The majority (>95%) of the gold nanoparticles are spherical.
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Do I need to wash my gold nanoparticles?
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For most applications, our gold nanoparticles can be used without any additional washing steps. If you have a sensitive application that requires additional washing the best way to do so is by either centrifugation or filtration.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do you attach gold nanoparticles to glass?
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To view the procedure on how to attach gold nanoparticles to glass please view information in the reference - Journal of Atomic, Molecular, and Optical PhysicsVolume 2012 (2012). Article ID 683830, 6 pagesdoi:10.1155/2012/683830
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Which gold nanoparticle size should I choose for my application?
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The size of gold nanoparticle to use is very depandent upon the intended application. Generally, smaller particles offer better sensitivity in applications such as immunogold labeling due to less steric hindrance and the ability to bind more gold nanoparticles to the desired target. Small gold nanoparticles are less visible than larger particles, however, which must also be taken into account.As an application example, particles with a size between 30-50nm are particularly useful for the development of rapid tests such as lateral flow assays.
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How do you attach gold nanoparticles to peptides and peptide conjugates?
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To view information on how to attach gold nanoparticles to peptides and peptide conjugates, please view the information in the reference - Rosenthal, S. J. and Wright, D. W. (Eds.). (2005), p. 91-92. NanoBiotechnology Protocols. New Jersey. Humana Press.
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