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762024

Sigma-Aldrich

Azide-PEG3-biotin conjugate

Synonym(s):

Polyethylene glycol, N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]hexahydro-2-oxo-(3aS,4S,6aR)- 1H-thieno[3,4-d ]imidazole-4-pentanamide, Biotin-PEG3-azide

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$75.10
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10 MG
$75.10
25 MG
$162.00
100 MG
$350.00

About This Item

Empirical Formula (Hill Notation):
C18H32N6O5S
CAS Number:
875770-34-6
Molecular Weight:
444.55
MDL number:
MFCD20134145
UNSPSC Code:
12352125
PubChem Substance ID:
329766598
NACRES:
NA.22

$75.10

List Price$81.10Save 7%
Web-Only Promotion

Available to ship onApril 22, 2025Details

Request a Bulk Order

form

solid

Quality Level

200

reaction suitability

reaction type: click chemistry

storage temp.

−20°C

SMILES string

O=C1N[C@](CS[C@H]2CCCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)([H])[C@]2([H])N1

InChI

1S/C18H32N6O5S/c19-24-21-6-8-28-10-12-29-11-9-27-7-5-20-16(25)4-2-1-3-15-17-14(13-30-15)22-18(26)23-17/h14-15,17H,1-13H2,(H,20,25)(H2,22,23,26)/t14-,15-,17-/m1/s1

InChI key

ZWFOOMQCYIGZBE-BFYDXBDKSA-N

Related Categories

Application

Azide-PEG3-biotin conjugate has been used for the detection of a cellular alkyne cholesterol analog via click chemistry using fluorescence microscopy after incubation with a fluorescent streptavidin-conjugate.[1] It has also been used to prepare a novel multifunctional benzophenone linker that can be used for pull-down assays, photoaffinity labeling and double-click stapling techniques.[2]
Biotinylation reagent for labeling alkyne containing molecules or biomolecules using either copper-catalyzed 1,3 dipolar cycloaddition click chemistry or copper-free click chemistry with cyclooctyne derivatives. The azide group reacts with alkynes to form a stable triazole linkage facilitating the introduction of biotin into your alkyne modified system of interest. This reaction is bioorthogonal or in other words compatible with biological systems in that it′s components do not react with the biological environment.

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCX3805
MKCV7845
MKCW7634
MKCW2338
MKCS5531

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Chapter Five-Mapping Lysine Acetyltransferase?Ligand Interactions by Activity-Based Capture
Montgomery DC & Meier JL
Methods in Enzymology, 574, 105-123 (2016)
Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N-and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars.
Woo CM, et al.
Analytical and Bioanalytical Chemistry, 409(2), 579-588 (2017)
A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization.
Hofmann K, et al.
Journal of Lipid Research, 55(3), 583-591 (2014)
Development of a Multifunctional Benzophenone Linker for Peptide Stapling and Photoaffinity Labelling
Wu Y, et al.
Chembiochem, 17(8), 689-692 (2016)
Xuan Ming et al.
Cell research, 30(11), 980-996 (2020-06-26)
Mitotic inheritance of the DNA methylome is a challenging task for the maintenance of cell identity. Whether DNA methylation pattern in different genomic contexts can all be faithfully maintained is an open question. A replication-coupled DNA methylation maintenance model was
View All Related Papers

Articles

Copper-Free Click Chemistry

Copper-free click chemistry is an alternative approach to click chemistry that proceeds at a lower activation barrier and is free of cytotoxic transition metal catalysts.

Click Chemistry in Drug Discovery

Explore the principles and applications of click chemistry in drug discovery, highlighting efficient reactions that streamline the synthesis of bioactive compounds.

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Polyethylene Glycol (PEG) Selection Guide

Polyethylene glycol (PEG), also sometimes referred to as polyethylene oxide (PEO), is a condensation polymer of ethylene oxide and water that has several chemical properties that make it useful for biological, chemical and pharmaceutical applications.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Can I aliquot Azide-PEG3-biotin after it is dissolved in buffer and store at -20deg? Is it stable?

    1 answer

    1. The solution stability of this product has not been determined internally. However, external sources state solutions of Biotin-PEG3-azide are stable at 80°C for 6 months and -20°C for 1 month.

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  4. Dear Sir or Madam, I ordered your compound Azide-PEG3-biotin and I just wanted to know what is the best solvent to resuspend it in?

    1 answer

    1. This product is not tested for solubility, however, other sources state that this material is soluble in DMSO, DMF, Acetonitrile, water, and PBS. The published rates are variable, but range from 40 mg/mL in DMSO and water, to 10 mg/ml in PBS. This information has not been validated.

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