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MilliporeSigma

767964

Cell Free Amino Acid Mixture - 13C,15N

5-100 mM in water, 98 atom % 15N, 98 atom % 13C

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About This Item

NACRES:
NA.12
UNSPSC Code:
12352209
Isotopic purity:
98 atom % 13C, 98 atom % 15N
Form:
liquid

Key Documents

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isotopic purity

98 atom % 13C, 98 atom % 15N

form

liquid

concentration

5-100 mM in water

technique(s)

bio NMR: suitable, protein expression: suitable

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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This Item
771031767972606839
Sigma-Aldrich

Sigma-Aldrich

767964

Cell Free Amino Acid Mixture - 13C,15N

Sigma-Aldrich

Sigma-Aldrich

771031

Cell Free Amino Acid Mixture-13C, 15N, D

Cell Free Amino Acid Mixture-15N 5-100 mM in water, 98 atom % 15N

Sigma-Aldrich

767972

Cell Free Amino Acid Mixture-15N

ISOGRO®-13C,15N Powder -Growth Medium 98 atom % 15N, 99 atom % 13C

Sigma-Aldrich

606839

ISOGRO®-13C,15N Powder -Growth Medium

technique(s)

bio NMR: suitable, protein expression: suitable

technique(s)

bio NMR: suitable, protein expression: suitable

technique(s)

bio NMR: suitable, protein expression: suitable

technique(s)

bio NMR: suitable, protein expression: suitable

isotopic purity

98 atom % 13C, 98 atom % 15N

isotopic purity

98 atom % 13C, 98 atom % 15N, 97 atom % D

isotopic purity

98 atom % 15N

isotopic purity

99 atom % 13C, 98 atom % 15N

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solid

form

solid

form

solid

form

solid

concentration

5-100 mM in water

concentration

2-100 mM in water

concentration

5-100 mM in water

concentration

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

This product suitable as source of Stable Isotope Labeled amino acids for Cell Free Protein Synthesis. For Mass Spec applications, consider product 909653, Stable Isotope Labeled Amino Acid Mixture for Mass Spec.

The product may appear turbid, due to the relatively high concentration of tyrosine. Once diluted for use, solution should be clear.

Packaging

1 mL aqueous solution of all 20 amino acids.
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Analysis Note

Amino acid concentrations vary by lot. Please refer to the specific lot Certificate of Analysis.

Approximate concentrations (mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20

used together

Product No.
Description
Pricing

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MBBD8875
MBBD8041
MBBD7937
MBBD4406
MBBD0850

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Estelle Couradeau et al.
Nature communications, 10(1), 2770-2770 (2019-06-27)
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare
Preparation of amino acid mixtures for cell-free expression systems
Caschera, F., Noireaux, V
Biotechniques, 58, 40-43 (2015)
Zhenzhen Liu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1124, 72-81 (2019-06-10)
A simple and fast LC-MS/MS method was developed and validated for simultaneous quantification of 20 proteinogenic l-amino acids (AAs) in a small volume (5 μL) of mouse plasma. Chromatographic separation was achieved on an Intrada Amino Acid column within 13 min via
Filippo Caschera et al.
BioTechniques, 58(1), 40-43 (2015-01-22)
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all
Sara Calhoun et al.
Communications biology, 4(1), 333-333 (2021-03-14)
Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus
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