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Sigma-Aldrich

In Vitro Protein Expression (iPE-SS) Kit for disulfide-containing proteins

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NACRES:
NA.12

usage

Stable Isotope Labeling
 extractions

Quality Level

technique(s)

bio NMR: suitable
protein expression: suitable

storage temp.

−70°C

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905089767824606839
vibrant-m

767824

iPE-Quick Kit

technique(s)

bio NMR: suitable, protein expression: suitable

technique(s)

bio NMR: suitable, protein expression: suitable

technique(s)

bio NMR: suitable, protein expression: suitable

technique(s)

bio NMR: suitable, protein expression: suitable

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

usage

Stable Isotope Labeling

usage

-

usage

-

usage

-

Application

This is a kit which is improved for the synthesis of extracellular secretory proteins such as cytokines and antibodies. This kit is suitable for the synthesis of stable isotope labeled disulfide-containing proteins.
This kit has been developed under license from RIKEN, incorporating their proprietary, advanced cell-free protein synthesizing technology into a kit dedicated to stable isotope labeling.

Packaging

Stable isotope labeled amino acid mixtures are not included in this kit. Please use the separately sold stable isotope labeled amino acids mixtures. (Prod. No. 767972, 767964, and 771031)
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Kit Components Also Available Separately

Product No.
Description
SDS

  • Internal Solution-SS

  • External Solution-SS

  • Control DNA (pUC-BAP)

  • L-Glutathione oxidized disodium salt

  • L-Glutathione reduced

  • Dialysis Cup

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Jun Yokoyama et al.
Analytical biochemistry, 411(2), 223-229 (2011-01-25)
During recent years, the targets of protein structure analysis using nuclear magnetic resonance spectroscopy have become larger and more complicated. As a result, a complete and precise stable isotope labeling technique has been desired. A cell-free protein synthesis system is
Takayoshi Matsuda et al.
Biochemical and biophysical research communications, 431(2), 296-301 (2013-01-08)
Many important therapeutic targets are secreted proteins with multiple disulfide bonds, such as antibodies, cytokines, hormones, and proteases. The preparation of these proteins for structural and functional analyses using cell-based expression systems still suffers from several issues, such as inefficiency

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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