803537

Bis(NHS)PEG5

• Synonym(s): Bis-N-succinimidyl-(pentaethylene glycol) ester, Bis-succinimide ester-activated PEG compound
• Empirical Formula (Hill Notation): C₂₂H₃₂N₂O₁₃
• CAS Number: 756526-03-1
• Molecular Weight: 532.50
• MDL number: MFCD11041118
• UNSPSC Code: 12352200
• NACRES: NA.22

Properties

• form: liquid
• Quality Level: 100
• mol wt: 532.5
• reaction suitability: reagent type: cross-linking reagent
• storage condition: desiccated
• solubility: water: soluble
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: N2(C(=O)CCC2=O)OC(=O)CCOCCOCCOCCOCCOCCC(=O)ON1C(=O)CCC1=O
• InChI key: FTYUGLBWKRXQBD-UHFFFAOYSA-N

Description

General description

By contrast to typical PEG reagents that contain heterogeneous mixtures of different PEG chain lengths, this PEG reagent is a homogeneous compound of defined molecular weight and spacer arm length, providing greater precision in optimization and characterization of crosslinking applications. Homobifunctional crosslinkers containing N-hydroxysuccinimide (NHS) esters are often used for low-resolution 3-D studies of protein structure and protein interaction analysis.

Features and Benefits

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds
• Water-soluble; compare to non-pegylated BS3 (also soluble) and DSS (water-insoluble)
• Membrane-impermeable, allowing for cell surface labeling
• Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
• Polyethylene glycol spacer arms help maintain conjugate solubility
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Ideal for small molecule or peptide conjugations

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable