Shen, Jinbo, et al. "Isolation, culture, and transient transformation of plant protoplasts
Shen J, et al.
Current Protocols in Cell Biology, 2014, 2-8 null
A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability
Lei R, et al.
Methods, 24-32 (2015)
Jennifer DeMars Tripp et al.
Yeast (Chichester, England), 30(5), 191-200 (2013-03-14)
Chemical-based methods have been developed for transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae with high efficiency. Transformation of early stationary-phase cells, e.g. cells grown in overnight liquid cultures or as colonies on plates, is less...
P D Drumheller et al.
Analytical biochemistry, 222(2), 380-388 (1994-11-01)
Polymer networks of poly(ethylene glycol) (PEG) in densely cross-linked matrices of acrylic acid (AA) and trimethylolpropane triacrylate were synthesized as biospecific cell adhesive substrates. Networks grafted with synthetic adhesion peptides produced substrates to investigate long-term, receptor-mediated cell/surface interactions, without nonspecific...
Ying-Chung Lin et al.
Nature protocols, 9(9), 2194-2205 (2014-08-22)
Isolated protoplasts serve as a transient expression system that is highly representative of stable transgenics in terms of transcriptome responses. They can also be used as a cellular system to study gene transactivation and nucleocytoplasmic protein trafficking. They are particularly...