81240

Sigma-Aldrich

Poly(ethylene glycol)

average Mn 4,000, platelets

Synonym(s):
PEG
Linear Formula:
H(OCH2CH2)nOH
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.23

Quality Level

form

platelets

mol wt

Mn 3,500-4,500
average Mn 4,000

mp

58-61 °C

Ω-end

hydroxyl

α-end

hydroxyl

storage temp.

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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General description

Polyethylene glycol (PEG) is a hydrophilic polymer. It can be easily synthesized by the anionic ring opening polymerization of ethylene oxide, into a range molecular weights and variety of end groups. When crosslinked into networks PEG can have high water content, forming “hydrogels PEG is a suitable material for biological applications because it does not trigger an immune response.

Application

Polyethylene glycol (PEG) platelets were used:
  • in PEG-directed gene delivery of protoplast transfection and incubation
  • to prepare extraction for measuring cytosolic phosphoenolpyruvate carboxykinase (PEPCK) from plant tissues
  • as DNA transformation agent in a study of transient gene expression of plant protoplasts, derived from rice leaf sheaths.
  • in preparing isothermal assembly buffer and TSS broth preparation for unique nucleotide sequence (UNS)-guided assembly of repetitive DNA parts
  • in transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae.
  • as DNA transformation reagent

Packaging

5 kg in glass bottle
1 kg in poly bottle

Other Notes

Polymer networks with grafted cell adhesion peptides for highly biospecific cell adhesive substrates

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Shen, Jinbo, et al. "Isolation, culture, and transient transformation of plant protoplasts
Shen J, et al.
Current Protocols in Cell Biology, 2014, 2-8 null
A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability
Lei R, et al.
Methods, 24-32 (2015)
Jennifer DeMars Tripp et al.
Yeast (Chichester, England), 30(5), 191-200 (2013-03-14)
Chemical-based methods have been developed for transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae with high efficiency. Transformation of early stationary-phase cells, e.g. cells grown in overnight liquid cultures or as colonies on plates, is less...
P D Drumheller et al.
Analytical biochemistry, 222(2), 380-388 (1994-11-01)
Polymer networks of poly(ethylene glycol) (PEG) in densely cross-linked matrices of acrylic acid (AA) and trimethylolpropane triacrylate were synthesized as biospecific cell adhesive substrates. Networks grafted with synthetic adhesion peptides produced substrates to investigate long-term, receptor-mediated cell/surface interactions, without nonspecific...
Ying-Chung Lin et al.
Nature protocols, 9(9), 2194-2205 (2014-08-22)
Isolated protoplasts serve as a transient expression system that is highly representative of stable transgenics in terms of transcriptome responses. They can also be used as a cellular system to study gene transactivation and nucleocytoplasmic protein trafficking. They are particularly...

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