900751

Biotinylated-D-lysine TFA salt

• Synonym(s): (R)-2-Ammonio-6-(5-((3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoate 2,2,2-trifluoroacetate, BDL TFA salt
• Empirical Formula (Hill Notation): C₁₆H₂₈N₄O₄S · C₂HF₃O₂
• Molecular Weight: 486.51
• NACRES: NA.22

Properties

• form: powder or crystals
• Quality Level: 100
• application(s): peptide synthesis
• storage temp.: −20°C
• SMILES string: O=C(O)C(F)(F)F.[O-]C([C@@H](CCCCNC(CCCC[C@@H]1[C@@](NC(N2)=O)([H])[C@@]2([H])CS1)=O)[NH3+])=O

Description

Application

Biotinylated D-lysine (BDL) can be used to conjugate a biotin handle into bacterial peptidoglycan (PG) for various applications. For example, Suzanne Walker and coworkers reported that penicillin-binding protein PBP4 of Staphylococcus aureus could use BDL to biotinylate Lipid I and Lipid II, facilitating assays to measure infamously low-abundance pools of cellular lipid-linked PG precursors. BDL provides a tool for studying other aspects of PG assembly including antibacterial development efforts.

Other Notes

Detection of Lipid-Linked Peptidoglycan Precursors by Exploiting an Unexpected Transpeptidase Reaction

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable