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MilliporeSigma

906913

Sigma-Aldrich

TissueFab® bioink 

Alg(Gel)ma -Vis/525 nm

Synonym(s):

Alginate, Bioink, GelMA-Alginate bioink, Gelatin methacrylamide, Gelatin methacrylate, Gelatin methacryloyl, Sodium alginate

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.23

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description

suitable for 3D bioprinting applications

form

gel form (Viscous)

impurities

≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)

color

colorless to pale yellow

pH

6.5-7.5

application(s)

3D bioprinting

storage temp.

2-8°C

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This Item
905429922862905410
application(s)

3D bioprinting

application(s)

3D bioprinting

application(s)

3D bioprinting

application(s)

3D bioprinting

pH

6.5-7.5

pH

6.5-7.5

pH

(6.5-7.5)

pH

6.5-7.5

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

gel form (Viscous)

form

viscous liquid

form

viscous liquid (gel)

form

viscous liquid

color

colorless to pale yellow

color

colorless to pale yellow

color

pale yellow to colorless

color

colorless to pale yellow

description

suitable for 3D bioprinting applications

description

0.2 μm sterile filtered, suitable for 3D bioprinting applications

description

Validation : HNMR at 40C

description

0.2 μm sterile filtered, suitable for 3D bioprinting applications

General description

3D bioprinting is the printing of biocompatible materials, cells, growth factors, and the other supporting materials necessary to yield functional complex living tissues. 3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.

Application

Gelatin methacrylate-Alginate bioink formulation is derived from natural polymers - gelatin and alginate. Gelatin contains bioactive peptide sequences similar to native extracellular matrix which promotes integrin mediated cell adhesion and MMP sensitive enzymatic degradation which are essential for cellular functions such as migration, proliferation and differentiation. Alginate is a natural polysaccharide widely used in tissue engineering, serves as a viscosity enhancer in the bioink formulation. TissueFab®- GelAlg-Vis bioink formulation is optimized for 3D bioprinting of tissues and constructs using any extrusion based 3D bioprinter. TissueFab® - GelAlg-Vis bioink formulation can be used to bioprint cell-laden hydrogels in desired shape and can be crosslinked by a combination of chemical crosslinking and visible light mediated photocrosslinking for further culture and maturation of cells for tissue engineering and regenerative medicine applications. Gelatin methacrylate bioinks have been used for 3D bioprinting with high printing resolution, shape fidelity and cell viability[1][2]. Gelatin methacrylate based bioinks have been used to bioprint osteogenic[3], chondrogenic[2][4], hepatic[1][5][6], adipogenic[7], vasculogenic[8], epithelial[6], endothelial[9][10], cardiac valve[11], skin[12], tumor[10] and other tissues and constructs. Gelatin and alginate containing bioinks have been used for bioprinting of 3D constructs with various cell types including human mesenchymal stem cells (hMSC)[2][8], embryonic stem cells (ESC)[13], human umbilical vein endothelial cells (HUVEC)[8][10], fibroblasts[10], cancer cells[10] etc.

Packaging

10 mL in glass bottle

Legal Information

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1 - STOT RE 2

target_organs

Liver,Upper respiratory tract

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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