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Low endotoxin gelatin solution

gel strength (bloom 300)

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Synonym(s):
Type A, Bloom 300

form

viscous liquid

Quality Level

concentration

20 wt. % in DPBS (buffer)

impurities

<25 EU/mL Endotoxin
<5 cfu/mL Bioburden

color

colorless to pale yellow

pH

6.5-7.5

storage temp.

2-8°C

Related Categories

Application

Low endotoxin gelatin solution is a 20% low endotoxin gelatin solution in DPBS buffer. It is sterile filtrated through 0.2 μm sterile filter, and ready to be used in biomedical applications.

Gelatin is widely used for tissue engineering and 3D bioprinting. Gelatin is derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. Gelatin solution has thermoreversible gelling property which enables synthesis of biocompatible and biodegradable hydrogels and promote cell adhesion, spreading, and proliferation.

Packaging

10 mL in glass bottle

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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J M Harlan et al.
Laboratory investigation; a journal of technical methods and pathology, 48(3), 269-274 (1983-03-01)
Lipopolysaccharide (LPS) produced time- and dose-dependent bovine endothelial cell injury in vitro that was manifested initially by cell detachment from culture substrate with subsequent cell lysis. Bovine endothelial cell injury was observed with LPS derived from Salmonella minnesota R595, a
K W Brunson et al.
Journal of supramolecular structure, 9(2), 231-242 (1978-01-01)
Chinese hamster ovary (CHO . K1 . PRO) cell growth was inhibited by addition of a gram-negative bacterial lipopolysaccharide (LPS) to the cell culture medium. Growth inhibition began after three or four days of incubation, was dose-dependent up to a
J Ongrádi et al.
Folia microbiologica, 29(6), 450-454 (1984-01-01)
Cytotoxicity of a mixed pyrogen preparation and its components as well as native and radiodetoxified lipopolysacharides (LPS) was determined with established HEp-2 cell cultures and by measuring plating efficiency. This proved to be more sensitive to the damaging effect of
E J Ziegler et al.
The New England journal of medicine, 307(20), 1225-1230 (1982-11-11)
In an effort to decrease deaths from gram-negative bacteremia and endotoxin shock, we treated bacteremic patients with human antiserum to endotoxin (lipopolysaccharide) core. Antiserum was prepared by vaccinating healthy men with heat-killed Escherichia coli J5; this mutant lacks lipopolysaccharide oligosaccharide
J G Brock-Utne et al.
Anaesthesia and intensive care, 17(1), 49-55 (1989-02-01)
Endotoxins (lipopolysaccharides, LPS) are potent bacterial poisons always present within the intestines in considerable amounts. Several pathophysiological conditions such as hypovolaemia, hypoxia, intestinal ischaemia, burns and radiation lead to a breakdown in the barrier and depending upon the extent of

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