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Sigma-Aldrich

NanoFabTx-DC-Chol Lipid Mix

for synthesis of cationic (DC-cholesterol) liposomes

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storage temp.

−20°C

General description

NanoFabTx-DC-Chol Lipid Mix; for synthesis of cationic (DC-cholesterol) liposomes is a ready-to-use nanoformulation blend for the synthesis of liposomes for drug delivery. The NanoFabTx-DC-Chol Lipid Mix includes optimized protocols with step-by-step instructions for synthesizing cationic DC-cholesterol liposomes for drug delivery applications. The modification of the liposomes with the cationic lipid, DC-cholesterol, have numerous advantages including high gene transfection efficiency. Liposome-based formulations are widely used for drug delivery applications and enable improved therapeutic efficacy of a range of drug types including small molecules, nucleic acids, proteins, and peptides.

Application

About NanoFabTx

NanoFabTx lipid mixes and formulation kits enable users to encapsulate a wide variety of therapuetic drug molecules for targeted or extended drug delivery without the need for lengthy trial-and-error optimization. NanoFabTx reagent kits provide an easy-to-use toolkit for encapsulating a variety of therapeutics in nanoparticles, microparticles, or liposomes. Drug encapsulated particles synthesized with the NanoFabTx kits are suitable for biomedical research applications such as oncology, immuno-oncology, gene delivery, and vaccine delivery.

Features and Benefits

  • A ready-to-use nanoformulation blend for the synthesis of cationic DC-cholesterol liposomes
  • Step-by-step protocols (extrusion or microfluidic) developed and tested by our formulation scientists
  • Flexible synthesis tools to create uniform and reproducible liposomes
  • Optimized to make liposomes around 100 nm with low polydispersity
  • DC-Cholesterol allows for high transfection efficiency and targeted drug delivery
Comprehensive protocols for liposome synthesis are included:
  • A lipid film hydration and extrusion protocol.
  • A microfluidics protocol using commercial platforms or syringe pumps.
The microfluidics protocol included with this product uses the NanoFabTx device kit (911593). These kits are ready-to-use and include the microfluidic chip, fittings, and tubing required for microfluidics-based synthesis (compatible microfluidics system or syringe pump required).

Legal Information

NANOFABTX is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Sterically stabilized liposomes: improvements in pharmacokinetics and antitumor therapeutic efficacy
Papahadjopoulos D, et al.
Proceedings of the National Academy of Sciences of the USA, 88, 11460-11464 (1991)
Which polymers can make nanoparticulate drug carriers long-circulating?
Torchilin VP, et al.
Advanced Drug Delivery Reviews, 16, 141?155-141?155 (1995)
Recent advances on liposomal nanoparticles: synthesis, characterization and biomedical applications
Panahi Y, et al.
Artificial Cells, Nanomedicine, and Biotechnology (Print), 45, 788-799 (2017)
Characterization of liposomal systems containing doxorubicin entrapped in response to pH gradients.
Mayer LD, et al.
Biochimica et Biophysica Acta, 1025, 143-151 (1990)
A L Klibanov et al.
FEBS letters, 268(1), 235-237 (1990-07-30)
Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the liposomes show

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