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Sigma-Aldrich

TissueFab® bioink 

(GelAlg)ma-Vis/405nm, low endotoxin

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Synonym(s):
Alginate, Alginate methacrylamide, Alginate methacrylate, Alginate methacryloyl, Bioink Low endotoxin AlgMA-LAP, Low endotoxin Alginate bioink, Sodium alginate

Quality Level

description

HNMR in D2O at 40 °C

sterility

0.2 μm filtered

form

viscous liquid (to gel)

impurities

<5 CFU/g Bioburden (Fungal)
<5 CFU/g Bioburden (Total Aerobic)
<50 EU/mL Endotoxin

color

pale yellow to colorless

pH

6.5-7.5

viscosity

15-60 cP(37 °C)

compatibility

3D bioprinting, extrusion bioprinting

storage temp.

2-8°C

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General description

TissueFab bioink -(GelAlg)ma-Vis/405nm, low endotoxin is an alginate methacrylate based bioink for 3D bioprinting applications. The formulation is optimized for 3D bioprinting of tissues and constructs using extrusion based 3D bioprinters. The bioink can be can be used to bioprint cell-laden hydrogels in your desired shape without any supporting material. The crosslinking of printed structures can be done in a single step using visible light mediated photocrosslinking. This bioink has applications in 3D cell culture, tissue engineering, and regenerative medicine.

This bioink is derived from natural polymers − alginate and gelatin. Alginate also known as sodium alginate or alginic acid, is a natural biopolymer obtained from brown algae. Alginate is a natural polysaccharide widely used in tissue engineering. Gelatin contains bioactive peptide sequences similar to native extracellular matrix which promotes integrin mediated cell adhesion and MMP sensitive enzymatic degradation which are essential for cellular functions such as migration, proliferation and differentiation.

Low Endotoxin, low bioburden: Endotoxins have been demonstrated negatively impact cellular growth, morphology, differentiation, inflammation and protein expression. Bioburden is defined as the number of contaminated organisms found in a given amount of material. We test each lot for endotoxins as well as total bioburden (aerobic and fungal) to minimize unwanted interactions. For more information: https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/microbiological-testing/pyrogen-testing/what-is-endotoxin

Packaging

10 mL in HDPE bottle

Legal Information

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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