MilliporeSigma
All Photos(3)

Documents

D199303

Sigma-Aldrich

2,4-Dinitrophenylhydrazine

reagent grade, 97%

All Photos(3)
Synonym(s):
(2,4-dinitrophenyl)-hydrazine, DNPH
Linear Formula:
(O2N)2C6H3NHNH2
CAS Number:
119-26-6
Molecular Weight:
198.14
Beilstein:
615586
MDL number:
MFCD00007578
PubChem Substance ID:
24893586
NACRES:
NA.21

grade

reagent grade

Assay

97%

contains

33% water (minimum)

mp

197-200 °C (lit.)

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
0473242195551945
2,4-Dinitrophenylhydrazine reagent grade, 97%

Sigma-Aldrich

D199303

2,4-Dinitrophenylhydrazine

2,4-Dinitrophenylhydrazine for HPLC derivatization, LiChropur™, moistened with 35% water, ≥99.0% (HPLC)

Supelco

04732

2,4-Dinitrophenylhydrazine

3,4-Dinitrophenol moistened with water, ≥97.0% (HPLC)

Sigma-Aldrich

42195

3,4-Dinitrophenol

2-Hydroxy-3,5-dinitropyridine 97%

Sigma-Aldrich

551945

2-Hydroxy-3,5-dinitropyridine

assay

97%

assay

≥99.0% (HPLC)

assay

≥97.0% (HPLC)

assay

97%

mp

197-200 °C (lit.)

mp

197-200 °C (lit.)

mp

130-135 °C

mp

175-179 °C (lit.)

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

100

contains

33% water (minimum)

contains

-

contains

~20% water as stabilizer

contains

-

General description

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis. The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).

Application

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.

Packaging

500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H206 - H302

Hazard Classifications

Acute Tox. 4 Oral - Desen. Expl. 1

Supplementary Hazards

EUH044

Storage Class Code

4.1A - Other explosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 1

1 of 1

2-Nitrophenylhydrazine 97%

Sigma-Aldrich

N21588

2-Nitrophenylhydrazine

J. Chem. Soc. Pak., 14, 73-73 (1992)
Clare L Hawkins et al.
Free radical biology & medicine, 46(8), 965-988 (2009-05-15)
Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of
Journal of the American Oil Chemists' Society, 70, 881-881 (1993)
Structure of 2, 4-dinitrophenylhydrazine.
Okabe N, et al.
Acta Crystallographica Section C, Crystal Structure Communications, 49(9), 1678-1680 (1993)
Chem. Abstr., 118, 22127d-22127d (1993)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service