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F2456

Fluorescein (free acid)

Dye content 95%, Powder

Fluorescein (free acid)
1 of 1 reviewers took part in a promotion
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2.5 g

Available to ship TODAYfromMILWAUKEE

$27.40
100 g

Available to ship TODAYfromMILWAUKEE

$48.90
500 g

Estimated to ship onApril 28, 2026fromMILWAUKEE

$193.00

About This Item

Empirical Formula (Hill Notation):
C20H12O5
CAS Number:
Molecular Weight:
332.31
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
45350:1
EC Number:
219-031-8
MDL number:
Beilstein/REAXYS Number:
94324

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Product Name

Fluorescein (free acid), Dye content 95 %

form

powder

Quality Level

composition

Dye content, 95%

technique(s)

titration: suitable

color

orange to red-brown

mp

320 °C (lit.)

λmax

496 nm

ε (extinction coefficient)

≥12000 at 282-286 nm, ≥39000 at 236-240 nm, ≥70000 at 488-492 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChI key

GNBHRKFJIUUOQI-UHFFFAOYSA-N

General description

Fluorescein (free acid, CI 45350) is a xanthene dye.[1] Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.

Application

Fluorescein (free acid) has been used as a staining agent in retinal imaging.[2]
Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

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1 of 1

This Item
F6377F7378216720
technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

color

orange to red-brown

color

-

color

white to faint yellow

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

powder

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Cunyang Wang et al.
Materials science & engineering. C, Materials for biological applications, 103, 109823-109823 (2019-07-28)
Acrylic bone cement (ABC) has been used as a grouting agent in joint replacement surgery for over 50 years. In particular, ABC is irreplaceable for high-load joint replacement such as total hip joint replacements (THJRs) and total knee joint replacements because
Tasuku Ueno et al.
Journal of the American Chemical Society, 126(43), 14079-14085 (2004-10-28)
Rational design strategies based on practical fluorescence modulation mechanisms would enable us to rapidly develop novel fluorescence probes for target molecules. Here, we present a practical and general principle for modulating the fluorescence properties of fluorescein. We hypothesized that (a)
Karen A Fisher et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 9(23), 5873-5878 (2003-12-16)
The uptake of the three species of the drug model fluorescein (fluorescein sodium salt (FNa), fluorescein free acid (F), and fluorescein diacetate (FDA)) by zeolite NaX and the mesoporous zeotype MCM-41 was investigated as well as their release rates into



Global Trade Item Number

SKUGTIN
F2456-2.5G04061835347650
F2456-500G04061833612927
F2456-100G04061835347643

Questions

Reviews

1 of 1 reviewers took part in a promotion

Active Filters

  1. Brown University Providence RI
    • Review 1
    • Votes 4
    1 out of 5 stars.

    new product not working when the old one did

    This product didn't work. i was wondering if we could be a credit or reimbursed?
    We have used this in the last (lot# BCCD8787) and it worked but the new one we got (BCCH4248) didn't.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for taking the time to leave a review. We are sorry to hear that your experience did not meet expectations. Our Customer Service team has reached out to you in effort to resolve this issue. We apologize for any inconvenience this may have caused.