All Photos(3)

F2456

Sigma-Aldrich

Fluorescein (free acid)

Dye content 95 %

Empirical Formula (Hill Notation):
C20H12O5
CAS Number:
Molecular Weight:
332.31
Colour Index Number:
45350:1
Beilstein:
94324
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder

composition

Dye content, 95%

mp

320 °C (lit.)

solubility

1 M NaOH: 1 mg/mL

λmax

496 nm

ε (extinction coefficient)

≥12000 at 282-286 nm
≥39000 at 236-240 nm
≥70000 at 488-492 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChI key

GNBHRKFJIUUOQI-UHFFFAOYSA-N

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General description

Fluorescein (free acid) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety. Principle involved in the modulation of its fluorescence properties has been described. Its uptake by zeolite NaX and the mesoporous zeotype MCM-41 has been investigated. It is widely employed as quantum yield standard.

Application

Fluorescein (free acid) has been used as a staining agent in retinal imaging.

Packaging

2.5, 100, 500 g in glass bottle

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Balamuralikrishna Jayaprakash et al.
Microbiome, 5(1), 138-138 (2017-10-17)
The limited understanding of microbial characteristics in moisture-damaged buildings impedes efforts to clarify which adverse health effects in the occupants are associated with the damage and to develop effective building intervention strategies. The objectives of this current study were (i)
Keisuke Kawamoto et al.
Haematologica, 103(6), 1018-1028 (2017-12-16)
The characteristics of adult patients with chronic active Epstein-Barr virus infection are poorly recognized, hindering early diagnosis and an improved prognosis. We studied 54 patients with adult-onset chronic active Epstein-Barr virus infection diagnosed between 2005 and 2015. Adult onset was
Ischemia-induced spreading depolarization in the retina.
Srienc AI
Journal of Cerebral Blood Flow and Metabolism, 36, 1579-1579 (2016)
Jodie L White et al.
Open forum infectious diseases, 6(7), ofz272-ofz272 (2019-07-10)
There are limited nationally representative data on correlates of cytomegalovirus (CMV) shedding among children and adolescents. In addition, the genotype distribution of CMV infections has not been well characterized among general populations in the United States. This study characterized urinary
Karen A Fisher et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 9(23), 5873-5878 (2003-12-16)
The uptake of the three species of the drug model fluorescein (fluorescein sodium salt (FNa), fluorescein free acid (F), and fluorescein diacetate (FDA)) by zeolite NaX and the mesoporous zeotype MCM-41 was investigated as well as their release rates into

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