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L023050

Sigma-Aldrich

DCA Deblock

configured for ÄKTA® and OligoPilot®, with Toluene

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Quality Level

type

for DNA synthesis
for DNA synthesis

product line

Proligo Reagents

storage condition

dry at room temperature

concentration

42.2-51.6 g/L (DCA content, titration)

impurities

≤200 ppm water content (Karl Fischer)

color

clear

compatibility

configured for ÄKTA® and OligoPilot®

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Preparation Note

Toluene / Dichloroacetic acid 97/3 (v/v)

Other Notes

Deblock solution containing dichloroacetic acid (DCA) in toluene removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Legal Information

OligoPilot is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Asp. Tox. 1 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT RE 2 Inhalation - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

39.2 °F

flash_point_c

4.0 °C


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