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L031100

Sigma-Aldrich

Activator 42®

0.25 M

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Synonym(s):
5-[3,5-Bis(trifluoromethyl)phenyl]-1H-tetrazole Solution, 5-[3,5-Bis(trifluoromethyl)phenyl]-2H-tetrazole Solution, Activator 42® Solution
CAS Number:

Quality Level

type

for DNA synthesis

product line

Proligo Reagents

form

liquid

storage condition

dry at room temperature

concentration

70-77 g/L (Activator 42 content, titration)

impurities

≤30 ppm water content (Karl Fischer)

color

clear

InChI

1S/C9H4F6N4/c10-8(11,12)5-1-4(7-16-18-19-17-7)2-6(3-5)9(13,14)15/h1-3H,(H,16,17,18,19)

InChI key

KKJFZIQEWZVVIV-UHFFFAOYSA-N

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1 of 4

This Item
L031180L031200L031000
Sigma-Aldrich

Sigma-Aldrich

L031100

Activator 42®

Sigma-Aldrich

Sigma-Aldrich

L031180

Activator 42®

Sigma-Aldrich

Sigma-Aldrich

L031200

Activator 42®

Sigma-Aldrich

Sigma-Aldrich

L031000

Activator 42®

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

dry at room temperature

color

clear

color

clear

color

clear

color

clear

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

for DNA synthesis

type

for DNA synthesis

type

for DNA synthesis

type

for DNA synthesis

product line

Proligo Reagents

product line

Proligo Reagents

product line

Proligo Reagents

product line

Proligo Reagents

Preparation Note

Acetronitrile / Activator 42 100 / 7 (v/w)

Other Notes

Activator solution, containing 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Legal Information

This product and it use is are covered under US Patent Number 7,897,758
Activator 42 is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

41.0 °F

Flash Point(C)

5 °C


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