L040031

Cap A

• UNSPSC Code: 12352200

Properties

• type: for DNA synthesis
• product line: Proligo Reagents
• concentration: 97.0-119.0 g/L (acetic anhydride, titration)
• color: clear colorlessto brownish
• Quality Level: 200

Description

Application

Cap A solution is used after the phosphoramidite reaction/coupling step.

Biochem/physiol Actions

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

General description

Cap A solution consists of acetic anhydrideanhydride, lutidine, and tetrahydrofuran (THF).

Safety Information

• pictograms: GHS02,GHS06,GHS08,GHS05
• signalword: Danger
• hcodes: H225,H302,H315,H318,H331,H335,H336,H351
• pcodes: P210 - P280 - P301 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• supp_hazards: EUH019
• Storage Class: 3 - Flammable liquids
• wgk: WGK 3