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L040150

Sigma-Aldrich

Cap 1

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Synonym(s):
Cap A
NACRES:
NA.21

type

for DNA synthesis

Quality Level

product line

Proligo Reagents

storage condition

dry at room temperature

impurities

≤200 ppm water content (Karl Fischer)

color

clear

suitability

conforms to structure (GC, Formulation)

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General description

Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).

Application

Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.

Biochem/physiol Actions

Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

35.6 °F - closed cup

Flash Point(C)

2.0 °C - closed cup


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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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