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M6250

2-Mercaptoethanol

≥99.0%

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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Size/SKUAvailabilityPrice
10 mL

Available to ship TODAYfromMILWAUKEE

$45.90
100 mL

Available to ship TODAYfromMILWAUKEE

$73.30
250 mL

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$92.80
500 mL

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$165.00
1 L

Available to ship TODAYfromMILWAUKEE

$203.00
2.5 L

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$322.00

About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
Assay:
≥99.0%
Bp:
157 °C (lit.)
Vapor pressure:
1 mmHg ( 20 °C)

$45.90


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vapor density

2.69 (vs air)

Quality Segment

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0%

form

liquid

expl. lim.

18 %

concentration

14.3 M (pure liquid)

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

functional group

hydroxyl, thiol

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

General description

2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Application

2-mercaptoethanol is widely used for retarding oxidation of biological compounds in solution.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

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This Item
M3148137151185469
description

≥99.0%

description

Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

description

EMPROVE® EVOLVE

description

ReagentPlus®, ≥99.0%, contains 50 ppm BHT as stabilizer

assay

≥99.0%

assay

99% (GC/titration)

assay

-

assay

≥99.0%

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

vapor pressure

1 mmHg ( 20 °C)

vapor pressure

1 mmHg ( 20 °C)

vapor pressure

1 mmHg ( 20 °C)

vapor pressure

6.17 mmHg ( 20 °C)

pH

4.5-6 (20 °C, 500 g/L)

pH

4.5-6 (20 °C, 500 g/L)

pH

-

pH

5.0-7.0 (25 °C)

refractive index

n20/D 1.500 (lit.)

refractive index

n20/D 1.500 (lit.)

refractive index

n20/D 1.500 (lit.)

refractive index

n20/D 1.402 (lit.)


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Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Target Organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

165.2 °F - closed cup

Flash Point (°C)

74 °C - closed cup

PPE (personal protective equipment)

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Can this bME be use in cell culture?

    1 answer
    1. This product has not been assessed for suitability in cell culture. Alternative product M3148, 2-Mercaptoethanol, is labeled as suitable for cell culture.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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