Nahoum G Anthony et al.
Journal of the American Chemical Society, 126(36), 11338-11349 (2004-09-10)
Isopropyl-thiazole ((iPr)Th) represents a new addition to the building blocks of nucleic acid minor groove-binding molecules. The DNA decamer duplex d(CGACTAGTCG)(2) is bound by a short lexitropsin of sequence formyl-PyPy(iPr)Th-Dp (where Py represents N-methyl pyrrole, (iPr)Th represents thiazole with an...
William Buchmann et al.
Journal of mass spectrometry : JMS, 44(8), 1171-1181 (2009-05-02)
A new method for the determination of the relative affinity of a ligand against various dsDNA sequences is presented by using electrospray ionization time-of-flight mass spectrometry (ESI-QTOF) mass spectrometry. The principle is described here through the complexation of double-stranded DNA...
Alexandre S Boutorine et al.
Nucleosides, nucleotides & nucleic acids, 26(10-12), 1559-1563 (2007-12-11)
Bis-conjugates of hairpin N-methylpyrrole/N-methylimidazole oligocarboxamide minor groove binders (MGB) possessing enhanced affinity and sequence-specificity for dsDNA were synthesized. Two hairpin MGBs were connected by their N-termini via an aminodiacetate linker. The binding of bis-MGB conjugates to the target DNA was...
Electrospray negative ionization mass spectrometry of polyamides containing N-methylpyrrole and N-methylimidazole.
Jun Wang et al.
Journal of mass spectrometry : JMS, 40(5), 688-689 (2005-01-28)
Gengo Kashiwazaki et al.
Bioorganic & medicinal chemistry, 17(3), 1393-1397 (2009-01-07)
We designed and synthesized alkylating conjugates 5-7 and their partner N-methylpyrrole-N-methylimidazole (PI) polyamides 8, 9. The DNA alkylating activities of conjugates 5-7 were evaluated by high-resolution denaturing polyacrylamide gel electrophoresis with a 219 base pair (bp) DNA fragment containing the...