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699800P

Avanti

MPLA (PHAD®)

Monophosphoryl Lipid A (Synthetic) (PHAD®), powder

Synonym(s):
PHAD phosphorylated hexaacyl disaccharide; Glycopyranoside Lipid A; GLA
Empirical Formula (Hill Notation):
C96H184N3O22P
CAS Number:
Molecular Weight:
1763.47
NACRES:
NA.25

assay

>99% (HPLC)

form

powder

packaging

pkg of 1 × 1 mg (699800P-1mg)
pkg of 1 × 5 mg (699800P-5mg)

manufacturer/tradename

Avanti Polar Lipids 699800P

shipped in

dry ice

storage temp.

−20°C

General description

Vaccination is well-accepted as an effective method to prevent infections by mounting pathogen-specific immune responses prior to the infection. Usually, immunization with vaccine antigens alone is not able to induce robust or long-lasting immune responses — resulting in failure of protective immunity against infections. Thus, adjuvants are required to enhance cellular or humoral immune responses upon immunization. Because vaccine adjuvants using Lipid A have proven to be safe and effective in inducing Th-1 type immune responses to heterologous proteins in animal and human vaccines, Avanti developed Phosphorylated HexaAcyl Disaccharide (PHAD®), the first fully synthetic monophosphoryl Lipid A available for use as an adjuvant in human vaccines.
Monophosphoryl lipid A (MPLA) is either extracted from bacterial lipid A or by chemical synthesis.

Application

MPLA PHAD® has been used:
  • as a component of cobalt porphyrin-phospholipid (Co-PoP) liposomes for the immunization of mice with membrane proximal external region (MPER) of the gp41 envelope protein
  • as an adjuvant along with dimethyldioctadecylammonium bromide(DDA) for C. muridarum recombinant membrane protein based multi-subunit vaccine
  • as toll-like receptor-4 (TLR4) agonist adjuvant for respiratory syncytial virus (RSV) fusion (F) protein FI-RSV vaccine

Biochem/physiol Actions

Monophosphoryl lipid A (MPLA) is a natural agonist for the toll-like receptor-4 (TLR4). It is useful as an adjuvant in immunization. MPLA is a safe prophylactic agent and has immunotherapeutic applications. MPLA used in vaccination improves B cell and T cell-mediated immunity.

Packaging

2 mL Amber Glass Crimp Cap Vial (699800P-5mg)
2 mL Amber Glass Crimp Cap Vial (699800P-1mg)

Other Notes

For R&D use only. Not for drug, household, or other uses.

Legal Information

PHAD is a registered trademark of Avanti Polar Lipids, Inc.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Rhea N Coler et al.
PloS one, 6(1), e16333-e16333 (2011-02-08)
Innate immune responses to vaccine adjuvants based on lipopolysaccharide (LPS), a component of gram-negative bacterial cell walls, are driven by Toll-like receptor (TLR) 4 and adaptor proteins including MyD88 and TRIF, leading to the production of inflammatory cytokines, type I
Christopher B Fox et al.
Sub-cellular biochemistry, 53, 303-321 (2010-07-02)
Natural derivatives and synthetic analogues of lipopolysaccharide are potent stimulators of the mammalian immune system. Retained adjuvant activity with reduced toxicity was obtained by the development of monophosphoryl lipid A (MPL((R))), which is approved for use in several vaccine products.
Caroline Boudousquié et al.
Vaccines, 8(1) (2020-01-18)
With the emergence of immune checkpoint inhibitors and adoptive T-cell therapies, there is a considerable interest in using personalized autologous dendritic cell (DC) vaccines in combination with T cell-targeting immunotherapies to potentially maximize the therapeutic impact of DC vaccines. Here
Construction of an Escherichia coli mutant producing monophosphoryl lipid A
Chen J, et al.
Biotechnology Letters, 33(5), 1013-1019 (2011)
Ryan C Anderson et al.
Colloids and surfaces. B, Biointerfaces, 75(1), 123-132 (2009-09-15)
Immunostimulatory molecules such as monophosphoryl lipid A (MPL), a Toll-like receptor 4 (TLR4) agonist, can be formulated to enhance vaccine adjuvant effects and to promote a Th1-type immune response. This study compares the in vitro and in vivo potency of

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