TopFluor® Cholesterol

23-(dipyrrometheneboron difluoride)-24-norcholesterol, powder

Bodipy Cholesterol; Bdp-Chol
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:


>99% (TLC)




pkg of 1 × 1 mg (810255P-1mg)
pkg of 1 × 10 mg (810255P-10mg)
pkg of 1 × 5 mg (810255P-5mg)


Avanti Polar Lipids 810255P

shipped in

dry ice

storage temp.


General description

Biomolecules attached to BODIPY, is readily absorbed in the visible light. It results in greater fluorescence quantum yields. It is highly photostable and less sensitive to change in polarity and pH.


TOPFLUOR®Cholesterol is suitable:
  • for cholesterol-uptake assay
  • for BODIPY cholesterol labeling in live cell experiments
  • for the formation of giant Janus liposomes via poly(vinyl alcohol) (PVA)-assisted lipid swelling

Biochem/physiol Actions

BODIPY labelled cholesterol has similar properties to that of the naïve cholesterol. This serves as a cholesterol marker for live cells and aids in trafficking intracellular cholesterol. It might also help in indicating lipid storage diseases.


5 mL Amber Glass Screw Cap Vial (810255P-5mg)
5 mL Amber Glass Screw Cap Vial (810255P-10mg)
5 mL Amber Glass Screw Cap Vial (810255P-1mg)

Legal Information

TopFluor is a registered trademark of Avanti Polar Lipids, Inc.


13 - Non Combustible Solids

WGK Germany


Certificate of Analysis

Certificate of Origin

Maarit Hölttä-Vuori et al.
Traffic (Copenhagen, Denmark), 9(11), 1839-1849 (2008-07-24)
Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with...
Frederik W Lund et al.
BMC biophysics, 5, 20-20 (2012-10-20)
Cholesterol is an important membrane component, but our knowledge about its transport in cells is sparse. Previous imaging studies using dehydroergosterol (DHE), an intrinsically fluorescent sterol from yeast, have established that vesicular and non-vesicular transport modes contribute to sterol trafficking...
James E Shaw et al.
Journal of structural biology, 155(3), 458-469 (2006-08-08)
Elucidating the role that charged membrane proteins play in determining cell membrane structure and dynamics is an area of active study. We have applied in situ correlated atomic force and confocal microscopies to characterize the interaction of the NAP-22 peptide...
Functional inhibition of acid sphingomyelinase disrupts infection by intracellular bacterial pathogens
Cockburn CL, et al.
Life science alliance, 2(2), e201800292-e201800292 (2019)
Silvia Scolari et al.
Biochemistry, 49(42), 9027-9031 (2010-09-25)
Seminal plasma proteins of the fibronectin type II (Fn2) family modulate mammalian spermatogenesis by triggering the release of the lipids phosphatidylcholine and cholesterol from sperm cells. Whereas the specific interaction of these proteins with phosphatidylcholine is well-understood, their selectivity for...

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