All Photos(2)

810255P

Avanti

TopFluor® Cholesterol

23-(dipyrrometheneboron difluoride)-24-norcholesterol, powder

Synonym(s):
Bodipy Cholesterol; Bdp-Chol
Empirical Formula (Hill Notation):
C36H51BF2N2O
CAS Number:
Molecular Weight:
576.61
NACRES:
NA.25

assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810255P-1mg)
pkg of 1 × 10 mg (810255P-10mg)
pkg of 1 × 5 mg (810255P-5mg)

shipped in

dry ice

storage temp.

−20°C

General description

Biomolecules attached to BODIPY, is readily absorbed in the visible light. It results in greater fluorescence quantum yields. It is highly photostable and less sensitive to change in polarity and pH.

Application

TOPFLUOR®Cholesterol is suitable:
  • for cholesterol-uptake assay
  • for BODIPY cholesterol labeling in live cell experiments
  • for the formation of giant Janus liposomes via poly(vinyl alcohol) (PVA)-assisted lipid swelling

Biochem/physiol Actions

BODIPY labelled cholesterol has similar properties to that of the naïve cholesterol. This serves as a cholesterol marker for live cells and aids in trafficking intracellular cholesterol. It might also help in indicating lipid storage diseases.

Packaging

5 mL Amber Glass Screw Cap Vial (810255P-5mg)
5 mL Amber Glass Screw Cap Vial (810255P-10mg)
5 mL Amber Glass Screw Cap Vial (810255P-1mg)

Legal Information

TopFluor is a registered trademark of Avanti Polar Lipids, Inc.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

Frederik W Lund et al.
BMC biophysics, 5, 20-20 (2012-10-20)
Cholesterol is an important membrane component, but our knowledge about its transport in cells is sparse. Previous imaging studies using dehydroergosterol (DHE), an intrinsically fluorescent sterol from yeast, have established that vesicular and non-vesicular transport modes contribute to sterol trafficking...
Maarit Hölttä-Vuori et al.
Traffic (Copenhagen, Denmark), 9(11), 1839-1849 (2008-07-24)
Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with...
Silvia Scolari et al.
Biochemistry, 49(42), 9027-9031 (2010-09-25)
Seminal plasma proteins of the fibronectin type II (Fn2) family modulate mammalian spermatogenesis by triggering the release of the lipids phosphatidylcholine and cholesterol from sperm cells. Whereas the specific interaction of these proteins with phosphatidylcholine is well-understood, their selectivity for...
Janus Liposomes: Gel-Assisted Formation and Bioaffinity-Directed Clustering
Wang M, et al.
Langmuir, 34(25), 7509-7518 (2018)
James E Shaw et al.
Biophysical journal, 90(6), 2170-2178 (2005-12-20)
Coupling atomic force microscopy (AFM) with high-resolution fluorescence microscopy is an attractive means of identifying membrane domains by both physical topography and fluorescence. We have used this approach to study the ability of a suite of fluorescent molecules to probe...

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