1α,25-Dihydroxyvitamin D3 solution

5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

Empirical Formula (Hill Notation):
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Quality Level


certified reference material




ampule of 1 mL




5 μg/mL in ethanol


single component solution

storage temp.


SMILES string




InChI key


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General description

A Certified Spiking Solution® suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS Vitamin D assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ. The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.
1α,25-Dihydroxyvitamin D3 is a biologically active form of vitamin D3 in calcium absorption and deposition. It has widespread effects on cellular differentiation and proliferation, and can modulate immune responsiveness, and central nervous system function. Recent studies suggest that 1α,25-dihydroxyvitamin D3 acts as a chemopreventive agent against several malignancies including cancers of the prostate and colon and shows synergy with other anticancer compounds.


1α,25-Dihydroxyvitamin D3 may be used as a certified reference material for the determination of the analyte in biological fluids by liquid chromatography-tandem mass spectrometry.

Legal Information

CERILLIANT is a registered trademark of Cerilliant Corporation
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Cerilliant Corporation


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Hazard Statements

Precautionary Statements


UN1170 - class 3 - PG 2 - Ethanol, solution

WGK Germany


Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14 °C - closed cup

Certificate of Analysis
Certificate of Origin
Determination of 1, 25-dihydroxyvitamin D2 and 1, 25-dihydroxyvitamin D3 in human serum using liquid chromatography with tandem mass spectrometry
Fang H, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 1027, 19-26 (2016)
Sensitive analysis of 1?, 25-dihydroxyvitamin D3 in biological fluids by liquid chromatography--tandem mass spectrometry
Kissmeyer AM and Sonne K
Journal of Chromatography A, 935(1-2), 93-103 (2001)
Zhican Wang et al.
Analytical biochemistry, 418(1), 126-133 (2011-07-26)
Simultaneous and accurate measurement of circulating vitamin D metabolites is critical to studies of the metabolic regulation of vitamin D and its impact on health and disease. To that end, we have developed a specific liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
Nicola Napoli et al.
PloS one, 8(2), e56488-e56488 (2013-02-26)
Vitamin D supplementation in childhood improves the achievement of peak bone mass. We investigated the effect of supplementation with calcitriol on bone turnover in recent-onset type 1 diabetes (T1D). Moreover, the association between osteocalcin and parameters of β-cell function and...
R Volchenkov et al.
Scandinavian journal of immunology, 77(4), 246-254 (2013-02-28)
Dendritic cells (DC) are professional antigen-presenting cells that are capable of both activating immune responses and inducing tolerance. Several studies have revealed efficiency of therapeutic vaccination with tolerogenic DC (tolDC) in inhibition of experimental autoimmunity. The purpose of this study...
Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.
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Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components
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Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep
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This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.
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Related Content
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity
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