1α,25-Dihydroxyvitamin D2 solution

5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

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Quality Level


certified reference material




ampule of 1 mL




5 μg/mL in ethanol


single component solution

storage temp.


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InChI key


General description

Accurate preparation of a quantitative reference is highly critical for accurate assessment of patient Vitamin D levels. This Certified Spiking Solution® is suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS Vitamin D assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ. The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.

Legal Information

CERILLIANT is a registered trademark of Cerilliant Corporation
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Cerilliant Corporation


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Precautionary Statements


UN1170 - class 3 - PG 2 - Ethanol, solution

WGK Germany


Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14 °C - closed cup

Certificate of Analysis
Certificate of Origin
Shigeaki Kato et al.
Current opinion in nephrology and hypertension, 16(4), 297-304 (2007-06-15)
Vitamin D has diverse biological actions, and consequently the mechanisms behind how it regulates gene transcription are diverse. Unlike its well described positive effects on gene transcription, little is known about how vitamin D induces transcriptional repression. Vitamin D-induced transcriptional...
D R Zimmerman et al.
Archives of biochemistry and biophysics, 392(1), 14-22 (2001-07-27)
Calcitroic acid (1 alpha-hydroxy-23 carboxy-24,25,26,27-tetranorvitamin D(3)) is known to be the major water-soluble metabolite produced during the deactivation of 1 alpha,25-dihydroxyvitamin D(3). This deactivation process involves a series of oxidation reactions at C(24) and C(23) leading to side-chain cleavage and...
Rachael M Biancuzzo et al.
The Journal of clinical endocrinology and metabolism, 98(3), 973-979 (2013-02-07)
The purpose of this study was to determine 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and 1,25-dihydroxyvitamin D2 [1,25(OH)2D2] levels in healthy adults consuming 1000 IU vitamin D2 or vitamin D3 per day for 11 weeks. Blood from 34 healthy male and female adults...
Frederick G Strathmann et al.
Clinical chemistry, 57(9), 1279-1285 (2011-07-20)
1α,25-dihydroxy vitamin D [1,25(OH)(2)D] is the active metabolite of vitamin D. Antibody-based detection methods lack specificity, but when combined with isotope dilution/ultra-performance liquid chromatography (UPLC)-tandem mass spectrometry, immunoextraction provides an attractive method for 1,25(OH)(2)D. We developed a method for simultaneous...
R A Henik et al.
Journal of the American Veterinary Medical Association, 215(11), 1644-1649 (2003-10-22)
Rickets was diagnosed in a 1-year-old cat with a history of weakness, osteopenia, and recurrent fractures. Processes causing rickets include vitamin D deficiency caused by inadequate, nutrition, lack of exposure to sunlight, defective metabolism of parent vitamin D to active...
Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.
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Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components
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Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep
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This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.
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Related Content
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity
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