MilliporeSigma
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03-0052-00

Millipore

SMC® Human Interleukin 4 (IL-4) Immunoassay Kit

eCl@ss:
32161000

Quality Level

species reactivity

human

manufacturer/tradename

SMC®

assay range

accuracy: 80-120%
(minimum of 10 samples spiked with acceptable recovery)

technique(s)

ELISA: suitable
single molecule counting: suitable

General description

Interleukin-4 (IL-4), is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets, and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
The SMC® Human IL-4 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.

Application

Research Sub Category
Cytokines & Cytokine Receptors
Used to detect/quantify:IL-4
The sensitivity of Single Molecule Counting (SMC®) gives the SMC® Human IL-4 Immunoassay Kit unparalleled performance in the quantification of IL-4 in human EDTA plasma.
Research Category
Inflammation & Immunology

Components

IL-4 Coated Beads
Standard Diluent
IL-4 Detection Antibody
Assay Buffer
IL-4 Standard
SMC® Human IL-4 Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D

Analysis Note

Low-end CV% Range: 2 - 12%|Low-end CV% Average: 7%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

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Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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