MilliporeSigma
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03-0089-01

Millipore

SMC® Human Interleukin 6 (IL-6) Immunoassay Kit

Synonym(s):
B-cell stimulatory factor 2 immunoassay kit, IL-6 ultra-sensitive immunoassay, IL-6 Single Molecule Counting Kit, IL 6 immunoassay kit
eCl@ss:
32161000

Quality Level

species reactivity

human

manufacturer/tradename

SMC®

assay range

accuracy: 80-120%
(minimum of 10 samples spiked with acceptable recovery)

technique(s)

ELISA: suitable
single molecule counting: suitable

General description

The SMC® IL-6 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
Interleukin-6 (IL-6) is a pro-inflammatory cytokine secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPs), which trigger the innate immune response and initiate inflammatory cytokine production. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also called a "myokine", a cytokine produced from muscle that increases in response to muscle contraction. Additionally, osteoblasts secrete IL-6 to stimulate osteoclast formation.

Application

Used to detect/quantify:IL-6
The sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® Human IL-6 Immunoassay Kit unparalleled performance in quantification of IL-6 in human EDTA plasma.
Research Sub Category
Cytokines & Cytokine Receptors
Research Category
Inflammation & Immunology

Components

Assay Buffer
IL-6 Coated Beads
Human IL-6 Standard Diluent
IL-6 Detection Antibody
Human IL-6 Standard
10X Wash Buffer
Buffer D
Elution Buffer B
SMC® Human IL-6 Immunoassay Kit Instructions

Analysis Note

Low-end CV% Range: 2 - 8%|Low-end CV% Average: 6%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

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Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificate of Analysis

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Certificate of Quality

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