The SMC® IL-6 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
Interleukin-6 (IL-6) is a pro-inflammatory cytokine secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPs), which trigger the innate immune response and initiate inflammatory cytokine production. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also called a "myokine", a cytokine produced from muscle that increases in response to muscle contraction. Additionally, osteoblasts secrete IL-6 to stimulate osteoclast formation.
Used to detect/quantify:IL-6
The sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® Human IL-6 Immunoassay Kit unparalleled performance in quantification of IL-6 in human EDTA plasma.
Research Sub Category
Cytokines & Cytokine Receptors
Inflammation & Immunology
IL-6 Coated Beads
Human IL-6 Standard Diluent
IL-6 Detection Antibody
Human IL-6 Standard
10X Wash Buffer
Elution Buffer B
SMC® Human IL-6 Immunoassay Kit Instructions
Low-end CV% Range: 2 - 8%|Low-end CV% Average: 6%
SMC is a registered trademark of Singulex
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