03-0153-00

SMC^® Glucagon High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Glucagon in human, rat or mouse plasma

• Synonym(s): Glucagon Single Molecule Counting Kit, Glucagon ultra-sensitive immunoassay, Pro-glucagon immunoassay kit
• NACRES: NA.84
• UNSPSC Code: 12161503

Properties

• Product Name: SMC^® Glucagon High Sensitivity Immunoassay Kit, Used for ultrasensitive quantification of Glucagon in human, rat or mouse plasma
• product line: SMC^®
• usage: kit sufficient for 96 wells
• quality: 200
• species reactivity: human, mouse, rat
• technique(s): single molecule counting: suitable
• input: plasma (K2 EDTA)
• UniProt accession no.: P01275
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

Application

Metabolism

Biochem/physiol Actions

Glucagon (human) was found to be 100% detectable.
Oxyntomodulin (human) was found to be <0.5% detectable.
GLP-1 (human) was not found to be detectable.
GLP-2 (human) was not found to be detectable.
GIP (human) was not found to be detectable.

Features and Benefits

• Ultra-Sensitive Detection — Quantifies glucagon down to 0.20 pg/mL
• Wide Dynamic Range — Covers 0.20–200 pg/mL
• SMC^® Technology — Enables single molecule resolution for precise quantification
• Species Compatibility — Validated for human, mouse, and rat plasma
• Detect Glucagon at Femto-Level Sensitivity — Ideal for metabolic and endocrine research
• Reliable Across Species — Supports translational studies from animal models to human samples
• Accurate Results with Minimal Interference — Trusted data in complex biological fluids

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 781 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 200-200,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Plasma: 86%
• Recovery: Plasma: 86%
• *Indicative LLOQ created from average of 14 independent in house runs,where >80% met same value defined with CV & Recovery +/- 20%. Refer toCoA for lot specific performance.

General description

Glucagon is a peptide hormone secreted by pancreatic alphacells that plays a vital role in maintaining glucose homeostasis. It acts as acounter-regulatory hormone to insulin by stimulating glycogenolysis andgluconeogenesis in the liver, thereby increasing blood glucose levels duringfasting or hypoglycemia. In addition to its role in glucose metabolism,glucagon also influences lipid metabolism and energy balance, making it a keybiomarker in metabolic and endocrine research.

Altered glucagon levels are associated with metabolicdisorders such as type 2 diabetes, obesity, and insulin resistance. Accuratemeasurement of glucagon is essential for understanding pancreatic function,evaluating therapeutic interventions, and studying the hormonal regulation ofenergy metabolism.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• target_organs: Respiratory Tract
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H312 + H332,H319,H360FD,H373,H412,EUH430
• pcodes: P201 - P202 - P273 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation