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03-0155-00

SMC® Human IL-6 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-6 in human serum and plasma

Synonym(s):

B-cell stimulatory factor 2 immunoassay kit, IL 6 immunoassay kit, IL-6 Single Molecule Counting Kit, IL-6 ultra-sensitive immunoassay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments

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Product Name

SMC® Human IL-6 High Sensitivity Immunoassay Kit,

storage temp.

2-8°C

description

Used for ultrasensitive quantification of Human IL-6 in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

P05231

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

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technique(s)

ELISA: suitable, single molecule counting: suitable

technique(s)

ELISA: suitable, single molecule counting: suitable

technique(s)

single molecule counting: suitable

technique(s)

ELISA: suitable, single molecule counting: suitable

species reactivity

human

species reactivity

guinea pig, nonhuman primates, rat, canine

species reactivity

mouse, feline, rat

species reactivity

mouse

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

assay range

sensitivity: 0.08 pg/mL
(lower limit of quantification)

assay range

-

assay range

sensitivity: 0.09 pg/mL
(Lower Limit of Quantification), inter-assay cv: 7%
intra-assay cv: 4%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)

assay range

-

General description

Interleukin 6 (IL-6) is secreted by T cells and macrophages and acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Some of its functions include the stimulation of the immune response during infection, induction of fever in autoimmune, infectious and non-infectious diseases, the stimulation of acute phase reactions, differentiation of T cells, proliferation and differentiation of B-cells, the development of blood cells as well as the activation of osteoclasts and osteoporosis and the secretion of vascular endothelium growth factor (VEFG) to increase blood vessel growth and vascular permeability in inflammation. It is important for a short-term defense against infection, injury and inflammation however dysregulation of IL-6 results in disease.

IL-6 is important in the study of diseases such as diabetes, lupus, multiple myeloma, rheumatoid arthritis and some cancers such as prostate and pancreatic cancer. Conversely, deficiency of IL-6 can have an effect on immune activation and IgA antibodies while overexpression of IL-6 can result in an immunological imbalance leading to an autoimmune pathology.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Ultra-Low Detection Limits: Enables femtogram-level detection of IL-6, far surpassing traditional ELISA sensitivity.
  • Expanded Dynamic Range: Reduces background noise, improving assay performance.
  • Targeted Research Applications: Ideal for sudies in inflammation, autoimmune diseases, and biomarker discovery.
  • Streamlined Lab Operations: Automation-ready reagents help optimize workflow and reduce manual handling.
  • Robust Performance: Supports preclinical and translational research with dependable results, enhancing quality of scientific investigations.
  • Advanced Technology: Powered by SMC®Technology for enhanced sensitivity.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 88 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 10-50,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 115% Plasma: 112%
  • Recovery: Serum: 112% Plasma: 100%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IL-6 was detectable in non-human primate (rhesus macaque and cynomolgus monkey) samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carolyne Pimentel Rosado et al.
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 46(12), 1469-1475 (2021-07-01)
This study evaluated the effect of green banana flour (GBF) consumption on obesity-related conditions in mice fed high-fat diets. GBF was prepared using stage 1 green banana pulp, which was dehydrated and milled. Mice were fed a control diet (n =

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