03-0156-00

SMC^® Human IL-13 High Sensitivity Immunoassay Kit

for quantification of IL-13 in human serum and plasma.

• Synonym(s): IL-13 Single Molecule Counting Kit, IL-13 ultra-sensitive immunoassay, Interleukin-13 immunoassay kit, NC30 immunoassay kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• species reactivity: guinea pig, nonhuman primates, rat, canine
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.04 pg/mL; (lower limit of quantification); inter-assay cv: <20%; intra-assay cv: <15%; (Intra-assay CV n=20; Inter-assay n=5)
• technique(s): ELISA: suitable; single molecule counting: suitable

Description

General description

Interleukin 13 (IL-13) is secreted by a number of cells including T helper cells, CD4 cells and a various white blood cells and is thought to be a mediator of allergic inflammation in many tissues via the induction of IgE synthesis by B cells. Although primarily associated with the induction of airway disease, IL-13 has also been shown to inhibit the production of proinflammatory cytokines by monocytes and macrophages suggesting it has important anti-inflammatory properties.

The SMC^® Human IL-13 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna and SMCxPRO immunoassay systems detect analytes using Single Molecule Counting (SMC) technology.

Specificity

Although IL-13 was detectable in non-human primate, rat, canine, and guinea pig samples, the assay has not been validated for these species.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Inflammatory Disease

Immune Response

Autoimmune disease

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC Human IL-13 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-13 in human serum and plasma.

This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-13 v2 kit, #03-0109-02:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Components

Assay Buffer

Human IL-13 Beads

Standard Diluent

Human IL-13 Detection Antibody

Human IL-13 Standard

10X Wash Buffer

Buffer D

Elution Buffer B

Specifications

Minimum of 5 serum samples spiked with average recovery of 90% with a range of 86-102% and 5 plasma samples spiked with average recovery of 92% with a range of 87-96%.

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• wgk_germany: WGK 3