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03-0162-00

Millipore

SMC® Human IL-22 High Sensitivity Kit

1 kit sufficient for 96 wells, input: serum, plasma

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Synonym(s):
IL-22 Single Molecule Counting Kit, IL-22 ultra-sensitive immunoassay, IL22 immunoassay kit, Interleukin-22 immunoassay kit

usage

 kit sufficient for 96 wells

species reactivity

human

manufacturer/tradename

SMC®

assay range

sensitivity: 0.10 pg/mL
(lower limit of quantification)

inter-assay cv: <20%
intra-assay cv: <15%
standard curve range: 0.05-50 pg/mL

technique(s)

ELISA: suitable
single molecule counting: suitable

input

serum
plasma

compatibility

for use with SMCxPRO® and Erenna<TMSYMBOL></TMSYMBOL> (validated)

shipped in

wet ice

storage temp.

2-8°C

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This Item
03-0157-0003-0190-0003-0160-00
species reactivity

human

species reactivity

human

species reactivity

mouse, feline, rat

species reactivity

human

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

assay range

sensitivity: 0.10 pg/mL
(lower limit of quantification), inter-assay cv: <20%
intra-assay cv: <15%
standard curve range: 0.05-50 pg/mL

assay range

sensitivity: 0.10 pg/mL
(lower limit of quantification), inter-assay cv: <20%
intra-assay cv: <15%
standard curve range: 0.10-100 pg/mL

assay range

sensitivity: 0.09 pg/mL
(Lower Limit of Quantification), inter-assay cv: 7%
intra-assay cv: 4%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)

assay range

sensitivity: 0.20 pg/mL
(lower limit of quantification), inter-assay cv: <20%
intra-assay cv: <15%
standard curve range: 0.05-50 pg/mL

technique(s)

ELISA: suitable, single molecule counting: suitable

technique(s)

ELISA: suitable, single molecule counting: suitable

technique(s)

single molecule counting: suitable

technique(s)

ELISA: suitable, single molecule counting: suitable

input

serum
plasma

input

serum
plasma

input

-

input

plasma
serum

General description

The SMC® human IL-22 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.

Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2)The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Specificity

Non-human primate plasma: 4 out of 4 individuals found to be detectable.
Feline plasma: 4 out of 4 individuals found to be detectable.
Canine plasma: 4 out of 4 individuals found to be detectable.
Mouse plasma: 5 out 6 individuals found to be detectable.
Although IL-22 was detectable, the assay has not been validated for these species.

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3


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Mahesh Raundhal et al.
Nature immunology, 22(4), 520-529 (2021-03-24)
Patients with myelodysplastic syndromes (MDSs) display severe anemia but the mechanisms underlying this phenotype are incompletely understood. Right open-reading-frame kinase 2 (RIOK2) encodes a protein kinase located at 5q15, a region frequently lost in patients with MDS del(5q). Here we

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