03-0177-00

SMC^® Human IL-17A/F Heterodimer High Sensitivity Immunoassay Kit

• Synonym(s): IL-17A/F Single Molecule Counting Kit, IL-17A/F ultra-sensitive immunoassay
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• description: 0.60-4.69 pg/mL, refer to CoA for lot-specific LLOQ
• species reactivity: monkey, canine, nonhuman primates
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 2.34 pg/mL; (Lower Limit of Quantification); inter-assay cv: <20%; intra-assay cv: <15%; (Intra-assay n=20; Inter-assay n=5)
• technique(s): single molecule counting: suitable
• storage temp.: 2-8°C

Description

Specificity

Species Reactivity:
Although IL-17A/F was detectable in non-human primate/monkey and canine, the assay has not been validated for these species.

Accuracy:

• Minimum of 5 serum samples spiked with average recovery of 101% with a range of 91-114%
• Minimum of 5 plasma samples spiked with average recovery of 88% with a range of 75-111%

Application

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human IL-17A/F High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-17A/F in human serum and plasma.

This new streamline kit has been improved with the following features including new including new improvements over the legacy IL-17A/F kit, #03-0119-00:

• Adjusted standard curve--changed to 0.3 – 300 pg/mL from 0.59 – 600 pg/mL
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage conditi
• Removal of a plate transfer ste• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Research Category:
Inflammation & Immunology

Research Sub-Category:

• Inflammatory Disease
• Immune Response
• Autoimmune disease

Packaging

96 well assay

Components

• Assay Buffer
• IL-17A/F Coated Beads
• Standard Diluent
• IL-17A/F Detection Antibody
• IL-17A/F Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects