03-0182-00
SMC® Human IL-12p70 High Sensitivity Immunoassay Kit
Synonym(s):
IL-12p70 Single Molecule Counting Kit, IL-12p70 ultra-sensitive immunoassay
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description
0.05-0.10 pg/mL, refer to CoA for lot-specific LLOQ
species reactivity
canine, rat, mouse, feline, monkey
manufacturer/tradename
SMC®
assay range
sensitivity: 0.1 pg/mL
(Lower Limit of Quantification)
inter-assay cv: <20%
intra-assay cv: <15%
(Inter-assay n=5; Intra-assay n=20)
technique(s)
single molecule counting: suitable
storage temp.
2-8°C
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This Item | 03-0177-00 | 03-0160-00 | 03-0156-00 |
|---|---|---|---|
| technique(s) single molecule counting: suitable | technique(s) single molecule counting: suitable | technique(s) ELISA: suitable, single molecule counting: suitable | technique(s) ELISA: suitable, single molecule counting: suitable |
| species reactivity canine, rat, mouse, feline, monkey | species reactivity monkey, canine, nonhuman primates | species reactivity human | species reactivity guinea pig, nonhuman primates, rat, canine |
| manufacturer/tradename SMC® | manufacturer/tradename SMC® | manufacturer/tradename SMC® | manufacturer/tradename SMC® |
| assay range sensitivity: 0.1 pg/mL | assay range sensitivity: 2.34 pg/mL | assay range sensitivity: 0.20 pg/mL | assay range - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
Application
This new streamline kit has been improved with the following features over the legacy IL-12 kit, #03-0057-00:
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
- Matched to WHO standard
Research Category:
Inflammation & Immunology
Research Sub-Category:
- Inflammatory Disease
- Immune Response
- Autoimmune disease
Biochem/physiol Actions
Although IL-12p70 was detectable in rat, mouse, monkey, canine, and feline plasma, the assay has not been validated for these species.
Cross Reactivity:
Cross-reactivity to interferons of other types was tested using SMC® Human IL-12p70 High Sensitivity Immunoasay Kit with the following results:
- rhIL-12p40 was found to be < 2.0% cross-reactive
- rhIL-23 was found to be < 1.5% cross-reactive
- rhILp35 was not found to be cross-reactive
Accuracy
- Minimum of 5 serum samples spiked with average recovery of 103% with a range of 100-106%
- Minimum of 5 plasma samples spiked with average recovery of 97% with a range of 84-102%
Packaging
Other Notes
- Assay Buffer
- IL-12p70 Coated Beads
- Standard Diluent
- IL-12p70 Detection Antibody
- IL-12p70 Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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