03-0185-00

SMC^® Human Total Tau High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human Total Tau in human serum, plasma, and cerebrospinal fluid (CSF)

• Synonym(s): Microtubile-associated protein tau immunoassay kit, Neurofibrillary tangle protein immunoassay kit, Tau Single Molecule Counting Kit, Tau ultra-sensitive immunoassay
• NACRES: NA.77
• UNSPSC Code: 12161503

Properties

• Product Name: SMC^® Human Total Tau High Sensitivity Immunoassay Kit, Used for ultrasensitive quantification of Human Total Tau in human serum, plasma, and cerebrospinal fluid (CSF)
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• input: plasma (K2 EDTA); cerebrospinal fluid (CSF); serum
• UniProt accession no.: P10636
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

• Sensitivity: LLOQ 4,690 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 1170-1,200,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 107% Plasma: 115% CSF: 103%
• Recovery: Serum 70% Plasma: 82% CSF: 91%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Application

Alzheimer′s Disease / Aging / Nervous System / Neurodegenerative Diseases / Neurofilament & Neuron Metabolism / Neuronal & Glial Markers / Neurodegenerative Medicine / Neuroscience

Biochem/physiol Actions

Although Total Tau was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

• Highly Sensitive Detection: Accurately measures Total Tau at femtogram/mL concentrations in serum, plasma or CSF.
• Enhanced Dynamic Range: Improved the signal-to-noise ratio with minimized background noise enables dependable assay results.
• Streamlined Workflow: Features a familiar sandwich immunoassay format to simplify adoption by researchers
• Automation Compatible: Designed to work with automated liquid handling systems, decreasing manual intervention and enhancing laboratory efficiency.
• Knowledgeable Technical Support: Supported by skilled field scientists and technical teams with expertise in SMC^® assays.

General description

Tau is a microtubule-associated protein predominantly found in neurons, where it stabilizes microtubules and supports axonal structure and function. By promoting microtubule assembly, tau facilitates intracellular transport and ensures effective communication between neurons. In neurological disorders, particularly neurodegenerative diseases such as Alzheimer′s disease, tau undergoes hyperphosphorylation, leading to its misfolding and aggregation into neurofibrillary tangles. These tangles disrupt microtubule stability, impair neuronal function, and contribute to cell death and cognitive decline.

Elevated tau levels in cerebrospinal fluid (CSF) often indicate ongoing neurodegeneration. Tau also plays a role in neuroinflammation; its presence can activate glial cells, which exacerbate inflammatory responses and promote further neuronal injury. Overall, tau is crucial for maintaining neuronal health, and its dysregulation is central to the pathogenesis of various neurodegenerative disorders.

Packaging

96 well assay

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• target_organs: Respiratory Tract
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H373,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation