03-0186-00
SMC® Human IFN-α2 High Sensitivity Immunoassay Kit
Synonym(s):
IFN-alpha-2 Single Molecule Counting Kit, IFN-alpha-2 immunoassay kit, IFN-alpha-2 ultra-sensitive immunoassay, Interferon alpha-A immunoassay kit
About This Item
Recommended Products
species reactivity
canine, feline, porcine, rat, nonhuman primates
manufacturer/tradename
SMC®
assay range
sensitivity: 0.062 pg/mL
(lower limit of quantification)
inter-assay cv: <13%
intra-assay cv: <9%
technique(s)
ELISA: suitable
single molecule counting: suitable
relevant disease(s)
autoimmune diseases
storage temp.
2-8°C
Related Categories
General description
The SMC® Human IFN-α2 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Specificity
This assay was tested for cross reactivity with rhIFN-β, rhIFN-γ, rhIFN-λ1 (IL-29), rhIFN-λ2 (IL28A), rhIFN-λ3 (IL-28B), rhIFN-ω, IFN-α2a, IFN-α2b and IFN-α.
- There is no cross-reactivity detected with rhIFN-ß, rhIFN-γ, rhIFN-λ1 (IL-29), rhIFN-λ2 (IL-28A),rhIFN-λ3 (IL-28B), and rhIFN-ω.
- There is 100% cross-reactivity with IFN-α2a.
- There is 49% cross-reactivity with IFN-α2b.
- There is 3% cross-reactivity with IFN-α.
Accuracy:
- Minimum of 5 serum samples spiked with average recovery of 102% with a range of 92-111%
- Minimum of 5 plasma samples spiked with average recovery of 96% with a range of 89-107%
Application
- Inflammation & Immunology
Research Sub-Category:
- Inflammatory Disease
- Immune Response
- Autoimmune disease
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human IFN-a2 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-a2 in human serum and plasma.
Features and Benefits
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Components
- Assay Buffer
- IFN-a2 Coated Beads
- Standard Diluent
- IFN-a2 Detection Antibody
- IFN-a2 Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Analysis Note
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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