03-0186-00

SMC^® Human IFN-α2 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IFNα2 in human serum and plasma

• Synonym(s): IFN-alpha-2 Single Molecule Counting Kit, IFN-alpha-2 immunoassay kit, IFN-alpha-2 ultra-sensitive immunoassay, Interferon alpha-A immunoassay kit
• NACRES: NA.84
• UNSPSC Code: 12161503
• Species reactivity: human
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Properties

• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• technique(s): single molecule counting: suitable
• input: serum; plasma (K2 EDTA)
• UniProt accession no.: P01563
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• relevant disease(s): autoimmune diseases
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Human Interferon alpha-2 (IFNa2) is a cytokine belonging to the IFN family exhibiting antiviral, antitumor and antiproliferative effects on immune response. IFNa2 is secreted by viral infected cells and acts to inhibit viral infection. It has been shown to play a role in autoimmune diseases such as lupus and myositis. The mechanisms of action underlying the pathogenic role that IFNa and IFNß play in autoimmunity is not well understood but elucidating their role will lead to a better understanding of these cytokines in chronic inflammation and immunity.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

• Sensitivity that sees what others miss: Captures cytokine fluctuations that standard ELISAs overlook, perfect for subtle immune shifts.
• Track Early Antiviral Responses: IFN-α2 is a key player in innate immunity; this kit helps detect its earliest signals in viral infection models.
• Advance Autoimmune Disease Research: Ideal for studying IFN-α2’s role in conditions like lupus, myositis, and other chronic inflammatory diseases.
• Wide Dynamic Range: Accurately quantifies IFN-α2 across a broad concentration spectrum, suitable for both baseline and elevated immune responses.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 60 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 15-80,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 90% Plasma: 102%
• Recovery: Serum: 102% Plasma: 96%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IFN-α2 was detectable in rat, pig, non-human primate, canine, and feline samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B