03-0190-00

SMC^® Human IL-18 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-18 in human serum and plasma

• Synonym(s): IGIF immunoassay kit, IL-18 Single Molecule Counting Kit, IL-18 ultra-sensitive immunoassay, Interleukin-18 immunoassay kit
• NACRES: NA.84
• UNSPSC Code: 12161503
• Species reactivity: human
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Properties

• Product Name: SMC^® Human IL-18 High Sensitivity Immunoassay Kit,
• description: Used for ultrasensitive quantification of Human IL-18 in human serum and plasma
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• technique(s): single molecule counting: suitable
• input: serum; plasma (K2 EDTA)
• UniProt accession no.: Q14116
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Interleukin-18 (IL-18) is a pro-inflammatory cytokine that plays a significant role in the immune response by promoting the activation and proliferation of T cells and natural killer (NK) cells. It is produced by macrophages and dendritic cells and is involved in the regulation of various immune functions, including the production of other cytokines such as interferon-gamma (IFN-γ). IL-18 contributes to the body′s defense against infections and is implicated in the pathogenesis of several inflammatory and autoimmune diseases.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

• Ultrasensitive SMC^® Technology: Detects IL-18 at femtogram-level concentrations, with a lower limit of quantification (LLOQ) around 90 fg/mL, making it ideal for low-abundance cytokine profiling.
• Wide Dynamic Range: Accurately quantifies IL-18 across a broad concentration spectrum, suitable for both baseline and elevated inflammatory states.
• Track Inflammatory Signaling with Precision: IL-18 plays a key role in innate immunity and inflammasome activation; this kit helps detect subtle shifts in its expression.
• Advance Research in Autoimmune and Infectious Diseases: Ideal for studying IL-18’s role in conditions like rheumatoid arthritis, Crohn’s disease, and viral infections.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 90 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 40-100,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 92% Plasma: 92%
• Recovery: Serum: 99% Plasma: 95%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IL-18 was detectable in mouse, rat, and feline samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B