03-0199-00

SMC^® Human NPTX2 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human NPTX2 in human serum, plasma and cerebrospinal fluid (CSF)

• Synonym(s): NPTX2 high sensitive immunoassay, Neuronal pentraxin 2 immuno assay kit, NPTX2 single molecule counting kit
• NACRES: NA.84
• UNSPSC Code: 12161503
• Species reactivity: human
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Properties

• Product Name: SMC^® Human NPTX2 High Sensitivity Immunoassay Kit,
• description: Used for ultrasensitive quantification of Human NPTX2 in human serum, plasma and cerebrospinal fluid (CSF)
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• technique(s): single molecule counting: suitable
• input: cerebrospinal fluid (CSF); serum; plasma (K2 EDTA)
• UniProt accession no.: P47972
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Neuronal Pentraxin 2 (NPTX2) actively contributes to synaptic function and plasticity in the brain. As a member of the pentraxin family, NPTX2 regulates synaptic transmission and clears excess neurotransmitters. Predominantly expressed in neurons, it facilitates the formation and maintenance of synapses, playing a crucial role in learning and memory. NPTX2 has been linked to conditions such as Alzheimer′s disease and schizophrenia. Altered levels of NPTX2 correlate with synaptic dysfunction and cognitive decline, suggesting its potential as a biomarker for these diseases. Additionally, NPTX2 influences neuroinflammatory processes by affecting interactions between neurons and glial cells. Understanding NPTX2′s function and its impact on synaptic integrity and neuroinflammation is essential for developing therapeutic strategies that aim to preserve cognitive function and mitigate the effects of neurodegeneration in various neurological disorders.

Application

NeuroscienceNeurological DisordersNeuronal & Glial Markers

Features and Benefits

• Ultrasensitive Detection: Detects NPTX2 at femtogram per milliliter levels in serum, plasma and CSF.
• Improved Signal Fidelity: Expanded dynamic range and higher signal-to-noise deliver consistent, accurate results.
• Familiar Sandwich Format: Uses a standard sandwich immunoassay that ELISA-trained labs can adopt easily while maintaining high sensitivity for Aβ1-42.
• Automation-compatible Design: Engineered for seamless integration with automated liquid-handling platforms to streamline workflows and reduce manual steps. Dedicated Technical Support: Backed by experts experienced in SMC^® assay technology.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 580 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 290-1,000,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 93% Plasma: 104% CSF: 97%
• Recovery: Serum: 99% Plasma: 104% CSF: 99%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although NPTX2 was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B