03-0204-00

SMC^® Human IgM High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IgM in human serum and plasma

• Synonym(s): IgM ultra-sensitive immunoassay, Immunoglobulin M immunoassay kit, IgM Single Molecule Counting kit, IgM immunoassay kit
• NACRES: NA.84
• UNSPSC Code: 12161503

Properties

• Product Name: SMC^® Human IgM High Sensitivity Immunoassay Kit, Used for ultrasensitive quantification of Human IgM in human serum and plasma
• Quality Level: 200
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• manufacturer/tradename: SMC^®
• technique(s): single molecule counting: suitable
• input: plasma (K2 EDTA); serum
• UniProt accession no.: P0DOX6
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments; for use with SMCxPRO^® and Erenna (validated)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

This assay does not show cross-reactivity against immunoglobulin subclasses IgA, IgD, IdE, IgG1, IgG2, IgG3, or IgG4 from in-house testing

Packaging

96 well assay

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

• Sensitivity: LLOQ 206,000 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 69,000-5,000,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 100% Plasma: 97%
• Recovery: Serum: 79% Plasma: 79%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

• Ultra-Sensitive Measurement: Enables detection of IgM down to fg/mL levels in diluted serum and plasma samples.
• Reliable Data: Enhanced dynamic range with improved signal-to-noise ratio ensures accurate and dependable results.
• Intuitive Assay Workflow: Follow a straightforward protocol to achieve high-sensitivity detection.
• Automated Workflow: Compatible with automated liquid handling systems to reduce manual steps and boosts laboratory productivity.
• Expert Support: Technical assistance is provided by specialized field scientists and technical teams.

General description

IgM (Immunoglobulin M) is the first antibody produced by the immune system in response to an infection or exposure to an antigen. It plays a critical role in the early stages of immune defense, providing a rapid response to pathogens. IgM is typically present in the bloodstream and is known for its ability to form pentamers, which enhances its capacity to bind to multiple antigens simultaneously and activate the complement system, leading to the destruction of pathogens.

Measuring IgM response is critical to vaccine development and infectious disease management. When a vaccine is administered, the body generates IgM antibodies as part of the initial immune response. Elevated IgM levels can indicate recent infections or acute immune responses, making it a valuable indicator of infection. An understanding of IgM immunogenicity is essential for evaluating vaccine efficacy and the immune response to infectious agents.

IgM also plays a significant role in assessing the safety of therapeutic proteins and other biologics, as it can signal potential hypersensitivity reactions. By studying IgM immunogenicity, researchers can identify risks associated with new treatments and enhance the design of vaccines and therapies to improve their effectiveness and minimize adverse effects.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B