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MilliporeSigma

04-259

Sigma-Aldrich

Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18

clone S5a-18, Upstate®, from mouse

Synonym(s):

S5a

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100 μL
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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

S5a-18, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002810.2
NM_153822.2

UniProt accession no.

P55036

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PSMD4(5710)

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SAB141112705-421MABE881
Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18 clone S5a-18, Upstate®, from mouse

Sigma-Aldrich

04-259

Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18

Anti-PSMD5 antibody produced in rabbit purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

SAB1411127

Anti-PSMD5 antibody produced in rabbit

Anti-PP2A Antibody, C subunit, clone 1D6 clone 1D6, Upstate®, from mouse

Sigma-Aldrich

05-421

Anti-PP2A Antibody, C subunit, clone 1D6

Anti-26S proteasome regulatory subunit RPN11, clone 7C7.1 Antibody clone 7C7.1, from mouse

Sigma-Aldrich

MABE881

Anti-26S proteasome regulatory subunit RPN11, clone 7C7.1 Antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

S5a-18, monoclonal

clone

polyclonal

clone

1D6, monoclonal

clone

7C7.1, monoclonal

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

rat, human

species reactivity

Saccharomyces cerevisiae, bovine, rabbit, rat, Xenopus, mouse, human

species reactivity

human, rat

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

Gene Information

human ... PSMD4(5710)

Gene Information

human ... PSMD5(5711)

Gene Information

human ... PPP2CA(5515)

Gene Information

human ... PSMD14(10213)

General description

41 kDa
Rpn10 (S5a) is a non-ATPase subunit of the 19S regulatory complex of the 26s proteasome. Subunit Rpn10 (S5a) of the 26S proteasome was originally identified as a protein capable of binding poly-ubiquitin chains of four or more ubiquitins, in the absence of other 26S proteasome subunits.

Immunogen

Full length human recombinant Rpn10 protein.

Application

Detect 19S regulator non-ATPase subunit S5a/Rpn10 using this Anti-19S regulator non-ATPase subunit S5a/Rpn10 Antibody, clone S5a-18 validated for use in IP & WB.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Biochem/physiol Actions

Recognizes 19S regulator non-ATPase subunit S5a/Rpn10.

Physical form

100 µL of purified immunoglobulin in PBS containing 0.01M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Format: Purified

Preparation Note

Stable for 1 year at -20°C from date of shipment for up to 12 months.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yuqing Lei et al.
STAR protocols, 3(1), 101035-101035 (2022-01-04)
Palmitoylation is a special kind of lipid modification that targets proteins to membranes. This protocol introduces the acyl-biotin exchange (ABE) assay to determine the palmitoylation of protein cysteines in yeast. Palmitoylation is exchanged by biotinylated compounds so that the palmitoyl proteins
Purification of poly-ubiquitinated proteins by S5a-affinity chromatography
Layfield, R, et al
Proteomics, 1, 773-777 (2001)
Anna J Slezak et al.
ACS central science, 8(10), 1435-1446 (2022-11-01)
Immune stimulating agents like Toll-like receptor 7 (TLR7) agonists induce potent antitumor immunity but are limited in their therapeutic window due to off-target immune activation. Here, we developed a polymeric delivery platform that binds excess unpaired cysteines on tumor cell
Kylie J Walters et al.
Biochemistry, 41(6), 1767-1777 (2002-02-06)
The 26S proteasome is essential for the proteolysis of proteins that have been covalently modified by the attachment of polyubiquitinated chains. Although the 20S core particle performs the degradation, the 19S regulatory cap complex is responsible for recognition of polyubiquitinated
C R Wilkinson et al.
Nature cell biology, 3(10), 939-943 (2001-10-05)
The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system. We describe the isolation of a fission-yeast gene, mud1+, which encodes a UBA domain containing protein that is able
View All Related Papers

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