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04-797

Sigma-Aldrich

Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39

clone Aw39, Upstate®, from rabbit

Synonym(s):
Mitogen-activated protein kinase, Extracellular signal-regulated kinase, protein tyrosine kinase ERK2
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

Aw39, monoclonal

species reactivity

human, rat

manufacturer/tradename

Upstate®

technique(s)

multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... MAPK1(5594)
rat ... Mapk1(116590)

General description

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).

Specificity

Predicted to cross-react with many species based on sequence homology.
Recognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.

Application

Research Category
Signaling
Research Sub Category
MAP Kinases
Western Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex microspheres. (Figure B).

Quality

Routinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells

Target description

MAPK1 (~44 kDa) and MAPK2 (~42 kDa).

Linkage

Replaces: 05-797

Physical form

Cultured supernantant in 0.05% sodium azide
Format: Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
RIPA cell lysate from NGF stimulated PC-12 cells

Other Notes

Concentration: Variable

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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