04-817
Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal
culture supernatant, clone MC463, Upstate®
Synonym(s):
H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μL | Estimated to ship TODAY | $700.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
MC463, monoclonal
Species reactivity:
human
Application:
ChIP, DB, ICC, IF, WB, multiplexing
Citations:
54
$700.00
Estimated to ship TODAYDetails
A recombinant, preservative-free antibody is available for your target. Try ZRB1226
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
MC463, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer10)
Gene Information
human ... HIST1H3F(8968)
General description
17kDa
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine
Immunogen
Peptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3
Application
Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified using DB & Mplex, published in peer reviewed journals & validated in ChIP, IF, WB, ICC, ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot Analysis:
A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).
Beadlyte Histone-Peptide Specificity Assay:
A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.
Immunofluorescence:
Representative image from a previous lot.
Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.
Chromatin Immunoprecipitation:
A previous lot was shown to be suitable for ChIP by an independent laboratory.
A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).
Beadlyte Histone-Peptide Specificity Assay:
A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.
Immunofluorescence:
Representative image from a previous lot.
Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.
Chromatin Immunoprecipitation:
A previous lot was shown to be suitable for ChIP by an independent laboratory.
Biochem/physiol Actions
Broad species cross-reactivity is expected
Histone H3 phosphorylated at Serine 10
Physical form
Cultured supernantant in 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts
routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells
Other Notes
Replaces: 05-817
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form culture supernatant | antibody form purified immunoglobulin | antibody form purified antibody | antibody form purified immunoglobulin |
| clone MC463, monoclonal | clone 3H10, monoclonal | clone 3H11, monoclonal | clone RR002, monoclonal |
| biological source rabbit | biological source mouse | biological source mouse | biological source mouse |
| species reactivity human | species reactivity human | species reactivity human | species reactivity vertebrates, nematode, Caenorhabditis elegans, human |
| Gene Information human ... HIST1H3F(8968) | Gene Information human ... HIST1H3F(8968) | Gene Information human ... HIST1H3F(8968) | Gene Information human ... HIST1H3F(8968) |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Heritable loss of replication control of a minichromosome derived from the B chromosome of maize.
Masonbrink, RE; Fu, S; Han, F; Birchler, JA
Genetics null
Mélanie Anne Hamon et al.
Infection and immunity, 79(7), 2839-2846 (2011-04-13)
Chromatin modification triggered by bacteria is a newly described mechanism by which pathogens impact host transcription. Listeria monocytogenes dephosphorylates histone H3 through the action of listeriolysin O (LLO); however, the underlying mechanism is unknown. Here we show that an unrelated
Aleksandr Vasilyev et al.
PloS one, 7(7), e39992-e39992 (2012-07-21)
Organ development leads to the emergence of organ function, which in turn can impact developmental processes. Here we show that fluid flow-induced collective epithelial migration during kidney nephron morphogenesis induces cell stretch that in turn signals epithelial proliferation. Increased cell
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 04-817 | 04053252404542 |