04-902
Anti-HA Tag Antibody, clone DW2, rabbit monoclonal
culture supernatant, clone DW2, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
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biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
DW2, monoclonal
species reactivity
vertebrates (common species)
technique(s)
western blot: suitable
isotype
IgG
shipped in
dry ice
target post-translational modification
unmodified
1 of 4
This Item | 05-904 | 05-902R | SAB4300603 |
|---|---|---|---|
| clone DW2, monoclonal | clone monoclonal | clone 114-2C-7, monoclonal | clone polyclonal |
| antibody form culture supernatant | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| biological source rabbit | biological source mouse | biological source rabbit | biological source rabbit |
| species reactivity vertebrates (common species) | species reactivity vertebrates (common species) | species reactivity - | species reactivity - |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| isotype IgG | isotype IgG3 | isotype IgG | isotype IgG |
General description
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Various
Immunogen
KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.
Application
Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.
Biochem/physiol Actions
Reactivity with other species has not been confirmed.
Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.
Physical form
Cultured supernatant in 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HA-tagged WNT-1 protein.
HA-tagged WNT-1 protein.
Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
Other Notes
Replaces: 05-902
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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