05-1000X

Anti-Phosphoserine Antibody, clone 4A4

clone 4A4, Upstate^®, from mouse

• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4A4, monoclonal
• species reactivity: vertebrates
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1
• shipped in: wet ice

Description

General description

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).

Specificity

Serine-phosphorylated proteins from all species

Immunogen

Phosphoserine coupled to KLH

Application

Anti-Phosphoserine Antibody, clone 4A4 is an antibody against Phosphoserine for use in WB, IF, FC, IH(P), ELISA.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Quality

Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.

Target description

Dependent upon the molecular weight of the serine phosphorylated protein being detected.

Physical form

50 µL of PBS with 0.05% sodium azide with 30% glycerol.

Format: Purified

Protein G-Sepharose Chromatography

Storage and Stability

2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2