05-100-I

Anti-HDAC1 Antibody, clone 2E10

clone 2E10, Upstate^®, from mouse

• Synonym(s): HDAC1, Histone deacetylase 1, HD1
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2E10, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_032254
• UniProt accession no.: O09106
• shipped in: wet ice
• Gene Information: human ... HDAC1(3065) ; mouse ... Hdac1(433759)

Description

General description

Histone deacetylase 1 is a nuclear enzyme encoded by the Hdac1 gene (also known as HD1, RPD3, MommeD5, Hdac1-ps) in mouse and HDAC1 (also known as GoON-10, RPD3L1) gene in human. Hdac1 plays an important role in epigenetic regulation of gene expression by catalyzing the hydrolysis of N(6)-acetylated histone lysine residues. Hdac1 is also known to deacetylate p53 and thereby downregulate p53 activity.

Immunogen

KLH-conjugated, synthetic peptide (C-EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.

Application

Anti-HDAC1 Antibody, clone 2E10 is an antibody against HDAC1 for use in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis A representative lot was used to immunoprecipitate HDAC1 from HeLa cell lysate, followed by immune complex enzyme activity assay using HDAC Assay Kit (Fluorometric Detection), Cat. No. 17-356.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HDAC1 in 10 µg of A431 cell lysate.

Target description

~55 kDa observed

Linkage

Replaces: 05-100

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable