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Anti-VEGF Antibody, clone VG1

ascites fluid, clone VG1, from mouse

Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165

Quality Level

biological source


antibody form

ascites fluid


VG1, monoclonal

species reactivity

rat, human, mouse


western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines.


Detects the 121, 165, and 189 amino acid isoforms of VEGF.


Recombinant human VEGF 189aa isoform


Detect VEGF using this Anti-VEGF Antibody, clone VG1 validated for use in WB.
Research Sub Category
Growth Factors & Receptors
Research Category


Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate.

Target description

Approx. 12-28 kDa


Replaces: MAB3734

Physical form

Unpurified mouse monoclonal IgG1κ in ascites fluid containing no preservatives.

Storage and Stability

Stable for 12 months at -20°C from date of receipt.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

NIH/3T3 cell lysate


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Emily C Dunford et al.
Journal of applied physiology (Bethesda, Md. : 1985), 122(3), 492-502 (2016-12-10)
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