05-117

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

clone bFM-1, Upstate^®, from mouse

• Synonym(s): Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2., GSK3 beta, GSK3B, Glycogen synthase kinase 3 beta, Glycogen synthase kinase 3β
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: bFM-1, monoclonal
• form: liquid
• mol wt: 17.5 kDa
• species reactivity: human, mouse, bovine, rat
• manufacturer/tradename: Upstate^®
• technique(s): neutralization: suitable; radioimmunoassay: suitable
• isotype: IgG1κ
• suitability: not suitable for Western blot
• NCBI accession no.: NM_002006.3
• UniProt accession no.: P09038
• shipped in: dry ice
• Gene Information: human ... FGF2(2247) ; mouse ... Fgf2(281161) , Fgf2(14173) ; rat ... Fgf2(54250)

Description

General description

Fibroblast growth factor 2 (FGF-2) belongs to the family of heparin-binding growth factors. It is localized in the cells of the mesoderm and neuroectodermal origin and several tumor cells. FGF-2 exists in four isoforms. It is made up of four cysteine residues, many basic residues, and two active sites. FGF-2 protein is a trigonal pyramidal structure formed by twelve anti-parallel β-sheets. The FGF-2 chromosome is located on the human chromosome at 4q28.1.

Research area: Cancer

Specificity

This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.

This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.

Immunogen

Epitope: a.a. 5-24

Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.

Application

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 has been used in:

• FGF2 neutralization assay (6 or 12 µg/mL)
• in vitro tube formation assay (1000 ng/mL)
• immunolabelling (1:200)

Biochem/physiol Actions

Fibroblast growth factor 2 (FGF-2) plays a role in vertebrate embryonic and fetal development. It acts as an inducer of angiogenesis and is a well-known mitogen (in vitro) for a variety of cells including fibroblasts and vascular endothelial cells. FGF-2 exerts its biological activity through high-affinity tyrosine kinase receptors and low-affinity heparan sulfate proteoglycans present on the cell surface. It exhibits autocrine effects on various cell functions involved in angiogenesis such as proliferation, integrin expression, migration, and proteinase production. FGF-2 participates in the growth, migration, differentiation, and survival of a variety of cells. The binding of FGF-2 with heparin protects FGF-2 from denaturation by heat and acid and proteolytic cleavage.

Quality

Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.

Target description

17.5 kDa

Physical form

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Fetal liver or kidney tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable