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05-1564

Sigma-Aldrich

Anti-mTOR Antibody, clone 21A12.2

clone 21A12.2, from mouse

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Synonym(s):
TORC2-specific protein AVO3, rapamycin-insensitive companion of mTOR
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

21A12.2, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MTOR(2475)

General description

mTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

Specificity

Other species to be tested.
This antibody only detects mTOR.

Immunogen

Full-length human mTOR

Application

Confocal Immunofluorescent Immunocytochemistry:
Detect mTOR using this Anti-mTOR Antibody, clone 21A12.2 validated for use in WB & IC.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

Evaluated by Western Blotting on HEK293 cell lysates.

Western Blotting Analysis: 1:1,000 - 2,000 dilution of this antibody was used to detect mTOR in HEK293 cell lysate.

Target description

~289 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
HEK293 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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