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Anti-mTOR Antibody, clone 21D8.2

clone 2ID8.2, from mouse

FK506 binding protein 12-rapamycin associated protein 1, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated prot

biological source


Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies


2ID8.2, monoclonal

species reactivity

mouse, human


immunocytochemistry: suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... MTOR(2475)

General description

mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.


This antibody recognizes mTOR.


Flag-tagged full-length mTOR protein


Anti-mTOR Antibody, clone 21D8.2 is an antibody against mTOR for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a previous lot detected mTOR in C2C12, HeLa, and NIH/3T3 cell lysates.
Research Category
Research Sub Category
PI3K, Akt, & mTOR Signaling


Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on HEK293 cell lysate.

Target description

289 kDa


Replaces: 04-385

Physical form

Format: Purified
Protein G Purified
Purified immunoglobulin containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl and 0.05% Sodium Azide.

Storage and Stability

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Analysis Note

Hek293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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